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dc:title "HTML Summary of #1824 \n\nPlant regeneration from embryogenic cell suspension cultures of wild sorghum (Sorghum dimidiatum Stapf.)\n\n";
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bibo:abstract "A simple and efficient protocol is described for regeneration of wild sorghum (Sorghum dimidiatum) from cell suspension cultures. Fast-growing cell suspensions were established from shoot-meristem-derived callus. Plating of the suspension on Murashige and Skoog agar medium supplemented with 2.5 mg 1-1 2,4-dichlorophen-oxyacetic acid (2,4-D) resulted in the formation of embryogenic calli. High-frequency (80%) somatic embryogenesis from small cell clusters (300-400 micrometer) was observed when the cultures were initially maintained in liquid medium with reduced levels of 2,4-D (0.25 mg l-1), followed by transfer to regeneration medium. Direct plating of these small clusters on regeneration medium or transfer to liquid regeneration medium containing kinetin and 6-benzylaminopurine resulted in the development of mature somatic embryos and plantlets. The regenerants developed to maturity and were all phenotypically and cytologically norma."^^xsd:string;
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bibo:issue "5";
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bibo:volume "18";
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,
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dct:date "1999";
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dct:title "Plant regeneration from embryogenic cell suspension cultures of wild sorghum (Sorghum dimidiatum Stapf.)"^^xsd:string;
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skos:prefLabel "Sorghum"@en .
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