%0 Journal Article
%A Mythili, P K
%A Seetharama, N
%A Reddy, V D
%D 1999
%F icrisat:1824
%I Springer Verlag
%J Plant Cell Reports
%N 5
%P 424-428
%T Plant regeneration from embryogenic cell suspension cultures of wild sorghum (Sorghum dimidiatum Stapf.)
%U http://oar.icrisat.org/1824/
%V 18
%X A simple and efficient protocol is described for regeneration of wild sorghum (Sorghum dimidiatum) from cell suspension cultures. Fast-growing cell suspensions were established from shoot-meristem-derived callus. Plating of the suspension on Murashige and Skoog agar medium supplemented with 2.5 mg 1-1 2,4-dichlorophen-oxyacetic acid (2,4-D) resulted in the formation of embryogenic calli. High-frequency (80%) somatic embryogenesis from small cell clusters (300-400 micrometer) was observed when the cultures were initially maintained in liquid medium with reduced levels of 2,4-D (0.25 mg l-1), followed by transfer to regeneration medium. Direct plating of these small clusters on regeneration medium or transfer to liquid regeneration medium containing kinetin and 6-benzylaminopurine resulted in the development of mature somatic embryos and plantlets. The regenerants developed to maturity and were all phenotypically and cytologically norma.