http://oar.icrisat.org/id/eprint/1824 2011-09-21T10:19:24Z info:eu-repo/semantics/descriptiveMetadata http://oar.icrisat.org/1824/ Plant regeneration from embryogenic cell suspension cultures of wild sorghum (Sorghum dimidiatum Stapf.) Mythili, P K Seetharama, N Reddy, V D Sorghum A simple and efficient protocol is described for regeneration of wild sorghum (Sorghum dimidiatum) from cell suspension cultures. Fast-growing cell suspensions were established from shoot-meristem-derived callus. Plating of the suspension on Murashige and Skoog agar medium supplemented with 2.5 mg 1-1 2,4-dichlorophen-oxyacetic acid (2,4-D) resulted in the formation of embryogenic calli. High-frequency (80%) somatic embryogenesis from small cell clusters (300-400 micrometer) was observed when the cultures were initially maintained in liquid medium with reduced levels of 2,4-D (0.25 mg l-1), followed by transfer to regeneration medium. Direct plating of these small clusters on regeneration medium or transfer to liquid regeneration medium containing kinetin and 6-benzylaminopurine resulted in the development of mature somatic embryos and plantlets. The regenerants developed to maturity and were all phenotypically and cytologically norma. Springer Verlag 1999 Article PeerReviewed application/pdf en http://oar.icrisat.org/1824/1/PlCellRep18%28424-428_1999.pdf Mythili, P K and Seetharama, N and Reddy, V D (1999) Plant regeneration from embryogenic cell suspension cultures of wild sorghum (Sorghum dimidiatum Stapf.). Plant Cell Reports, 18 (5). pp. 424-428. http://dx.doi.org/10.1007/s002990050597 info:eu-repo/semantics/objectFile info:eu-repo/semantics/humanStartPage