@article{icrisat1824,
           title = {Plant regeneration from embryogenic cell suspension cultures of wild sorghum (Sorghum dimidiatum Stapf.)},
       publisher = {Springer Verlag},
          author = {P K Mythili and N Seetharama and V D Reddy},
           pages = {424--428},
            year = {1999},
          volume = {18},
         journal = {Plant Cell Reports},
          number = {5},
             url = {http://oar.icrisat.org/1824/},
        abstract = {A simple and efficient protocol is described for regeneration of wild sorghum (Sorghum dimidiatum) from cell suspension cultures. Fast-growing cell suspensions were established from shoot-meristem-derived callus. Plating of the suspension on Murashige and Skoog agar medium supplemented with 2.5 mg 1-1 2,4-dichlorophen-oxyacetic acid (2,4-D) resulted in the formation of embryogenic calli. High-frequency (80\%) somatic embryogenesis from small cell clusters (300-400 micrometer) was observed when the cultures were initially maintained in liquid medium with reduced levels of 2,4-D (0.25 mg l-1), followed by transfer to regeneration medium. Direct plating of these small clusters on regeneration medium or transfer to liquid regeneration medium containing kinetin and 6-benzylaminopurine resulted in the development of mature somatic embryos and plantlets. The regenerants developed to maturity and were all phenotypically and cytologically norma.}
}