eprintid: 1823
rev_number: 11
eprint_status: archive
userid: 14
dir: disk0/00/00/18/23
datestamp: 2011-09-21 10:17:50
lastmod: 2013-09-14 09:34:49
status_changed: 2011-09-21 10:17:50
type: article
metadata_visibility: show
contact_email: Library-ICRISAT@cgiar.org
item_issues_count: 0
creators_name: Naidu, R A
creators_name: Kimmins, F M
creators_name: Holt, J
creators_name: Robinson, D J
creators_name: Deom, C M
creators_name: Subrahmanyam, P
icrisatcreators_name: Naidu, R A
icrisatcreators_name: Subrahmanyam, P
affiliation: ICRISAT(Lilongwe)
affiliation: Natural Resources Institute
affiliation: Scottish Crop Research Institute
affiliation: University of Georgia
country: Malawi
country: UK
title: Spatiotemporal Separation of Groundnut Rosette Disease Agents
ispublished: pub
subjects: s1.3
full_text_status: public
abstract: Analysis by triple-antibody sandwich enzyme-linked immunosorbent assay of groundnut samples from fields in two seasons from different regions of Malawi showed the absence of groundnut rosette assistor virus (GRAV) from some plants showing groundnut rosette disease symptoms and the presence of GRAV in some symptomless plants. Viruliferous Aphis craccivora collected from fields transmitted either GRAV alone, groundnut rosette virus (GRV) with its satellite RNA (sat RNA), or all three agents together, in different proportions. More plants became infected with all three agents when increasing numbers of potentially viruliferous aphids were used per plant, suggesting a dosage response. Electrical penetration graph studies of aphid stylet activities indicated successful transmission of GRV and its sat RNA during both the "stylet pathway phase" and salivation into sieve elements, whereas GRAV was transmitted only during the latter phase. Aphids transmitted all three agents together only during the salivation phase. Reverse-transcriptase polymerase chain reaction testing of viruliferous aphids and of inoculated plants revealed no correlation between the presence of all three agents in prospective aphid vectors and their simultaneous transmission to groundnut plants. These results show that separation of the groundnut rosette disease agents occurs over time and space.
date: 1999
publication: Phytopathology
volume: 89
number: 10
pagerange: 934-941
refereed: TRUE
official_url: http://dx.doi.org/10.1094/PHYTO.1999.89.10.934
related_url_url: http://scholar.google.co.in/scholar?as_q=Spatiotemporal+Separation+of+Groundnut+Rosette+Disease+Agents&num=10&btnG=Search+Scholar&as_epq=&as_oq=&as_eq=&as_occt=title&as_sauthors=&as_publication=&as_ylo=&as_yhi=&as_sdt=1.&as_sdtp=on&as_sdtf=&as_sdts=5&hl=e
related_url_type: author
citation:   Naidu, R A and Kimmins, F M and Holt, J and Robinson, D J and Deom, C M and Subrahmanyam, P  (1999) Spatiotemporal Separation of Groundnut Rosette Disease Agents.  Phytopathology, 89 (10).  pp. 934-941.      
document_url: http://oar.icrisat.org/1823/1/Phytopathology89_10_934-941_1999.pdf