OAR@ICRISATA simple method for long-term preservation of cultures of Colletotrichum graminicola and C. gloeosporioides [Glomeralla cingulata] causing anthracnose in sorghumKMathurauthorR PThakurauthorV PRaoauthorLong-term preservation and maintenance of pathogen isolates is essential for studies on pathogenic variability and virulence monitoring. Once the pathogen isolates are appropriately characterized for their morphologic, pathogenic, and genetic diversity they become reference isolates for future studies. Frequent subculturing of necrotrophic pathogens is not only laborious, but it can also induce spontaneous natural mutations in actively growing cultures. There are numerous reports of reduced viability and pathogenicity of isolates due to repeated subculturing on synthetic media. In our studies on pathogenic variability in Colletotrichum graminicola (Ces.) Wils. and C. gloeosporioides (Penz.) Sacc., the causal agents of anthracnose of sorghum (Mathur et al. 1998), we faced the problem of adequately maintaining a large number of isolates (>200) by repeated subculturing on oatmeal agar (OMA) at monthly intervals.Sorghum1999Article

For work being deposited by its own author: In self-archiving this collection of files and associated bibliographic metadata, I grant OAR@ICRISAT the right to store them and to make them permanently available publicly for free on-line. I declare that this material is my own intellectual property and I understand that OAR@ICRISAT does not assume any responsibility if there is any breach of copyright in distributing these files or metadata. (All authors are urged to prominently assert their copyright on the title page of their work.)

For work being deposited by someone other than its author: I hereby declare that the collection of files and associated bibliographic metadata that I am archiving at OAR@ICRISAT) is in the public domain. If this is not the case, I accept full responsibility for any breach of copyright that distributing these files or metadata may entail.

Clicking on the deposit button indicates your agreement to these terms.