eprintid: 1542 rev_number: 10 eprint_status: archive userid: 14 dir: disk0/00/00/15/42 datestamp: 2011-09-27 05:25:42 lastmod: 2011-09-27 05:25:42 status_changed: 2011-09-27 05:25:42 type: article metadata_visibility: show contact_email: Library-ICRISAT@cgiar.org item_issues_count: 0 creators_name: Mace, E S creators_name: Godwin, I D icrisatcreators_name: Mace, E S affiliation: ICRISAT(Patancheru) affiliation: University of Queensland(Brisbane) country: India country: Australia title: Development and characterization of polymorphic microsatellite markers in taro (Colocasia esculenta) ispublished: pub subjects: s2.13 full_text_status: restricted note: The authors gratefully acknowledge the support of the Australian Centre for International Agricultural Research (ACIAR) project CS2/94/43, and the collaboration received through the AusAID-funded Taro Genetic Resources project. Additonally, the technical support provided by Dr. Giovanni Cordeiro and Dr. Tina Maguire is gratefully acknowledged. abstract: Microsatellite-containing sequences were isolated from enriched genomic libraries of taro (Colocasia esculenta (L.) Schott). The sequencing of 269 clones yielded 77 inserts containing repeat motifs. The majority of these (81.7%) were dinucleotide or trinucleotide repeats. The GT/CA repeat motif was the most common, accounting for 42% of all repeat types. From a total of 43 primer pairs designed, 41 produced markers within the expected size range. Sixteen (39%) were polymorphic when screened against a restricted set of taro genotypes from Southeast Asia and Oceania, with an average of 3.2 alleles detected on each locus. These markers represent a useful resource for taro germplasm management, genome mapping, and marker-assisted selection. date: 2002 date_type: published publication: Genome volume: 45 number: 5 publisher: National Research Council Canada pagerange: 823-832 refereed: TRUE issn: 1480-3321 official_url: http://dx.doi.org/10.1139/G02-045 related_url_url: http://scholar.google.co.in/scholar?as_q=Development+and+characterization+of+polymorphic+microsatellite+markers+in+taro+%28Colocasia+esculenta%29&num=10&btnG=Search+Scholar&as_epq=&as_oq=&as_eq=&as_occt=title&as_sauthors=&as_publication=&as_ylo=&as_yhi=&a related_url_type: author funders: Australian Centre for International Agricultural Research citation: Mace, E S and Godwin, I D (2002) Development and characterization of polymorphic microsatellite markers in taro (Colocasia esculenta). Genome, 45 (5). pp. 823-832. ISSN 1480-3321 document_url: http://oar.icrisat.org/1542/1/Genome45_5_823-832_2002.pdf