%O The authors gratefully acknowledge the support of the
Australian Centre for International Agricultural Research
(ACIAR) project CS2/94/43, and the collaboration received
through the AusAID-funded Taro Genetic Resources project.
Additonally, the technical support provided by Dr. Giovanni
Cordeiro and Dr. Tina Maguire is gratefully acknowledged.
%A E S Mace
%A I D Godwin
%I National Research Council Canada
%V 45
%L icrisat1542
%J Genome
%N 5
%P 823-832
%D 2002
%X Microsatellite-containing sequences were isolated from enriched genomic libraries of taro (Colocasia esculenta (L.) Schott). The sequencing of 269 clones yielded 77 inserts containing repeat motifs. The majority of these (81.7%) were dinucleotide or trinucleotide repeats. The GT/CA repeat motif was the most common, accounting for 42% of all repeat types. From a total of 43 primer pairs designed, 41 produced markers within the expected size range. Sixteen (39%) were polymorphic when screened against a restricted set of taro genotypes from Southeast Asia and Oceania, with an average of 3.2 alleles detected on each locus. These markers represent a useful resource for taro germplasm management, genome mapping, and marker-assisted selection.
%T Development and characterization of polymorphic microsatellite markers in taro (Colocasia esculenta)