TY - JOUR AV - restricted A1 - Mace, E S A1 - Godwin, I D TI - Development and characterization of polymorphic microsatellite markers in taro (Colocasia esculenta) UR - http://dx.doi.org/10.1139/G02-045 JF - Genome SN - 1480-3321 PB - National Research Council Canada N1 - The authors gratefully acknowledge the support of the Australian Centre for International Agricultural Research (ACIAR) project CS2/94/43, and the collaboration received through the AusAID-funded Taro Genetic Resources project. Additonally, the technical support provided by Dr. Giovanni Cordeiro and Dr. Tina Maguire is gratefully acknowledged. N2 - Microsatellite-containing sequences were isolated from enriched genomic libraries of taro (Colocasia esculenta (L.) Schott). The sequencing of 269 clones yielded 77 inserts containing repeat motifs. The majority of these (81.7%) were dinucleotide or trinucleotide repeats. The GT/CA repeat motif was the most common, accounting for 42% of all repeat types. From a total of 43 primer pairs designed, 41 produced markers within the expected size range. Sixteen (39%) were polymorphic when screened against a restricted set of taro genotypes from Southeast Asia and Oceania, with an average of 3.2 alleles detected on each locus. These markers represent a useful resource for taro germplasm management, genome mapping, and marker-assisted selection. Y1 - 2002/// SP - 823 ID - icrisat1542 EP - 832 VL - 45 IS - 5 ER -