http://oar.icrisat.org/id/eprint/132 2011-10-24T03:42:25Z info:eu-repo/semantics/descriptiveMetadata http://oar.icrisat.org/132/ Cajanus platycarpus (Benth.) Maesen as the donor of new pigeonpea cytoplasmic male sterile (CMS) system Mallikarjuna, N Jadhav, D R Srikant, S Saxena, K B Pigeonpea Cajanus platycarpus, a distantly related wild species, was successfully crossed with cultivated pigeonpea using embryo rescue and tissue culture techniques. Advance generation lines showed a range of desirable characters including cytoplasmic male sterility. A range of pigeonpea cultivars restored fertility and was maintained by a few lines including cultivar ICPL 85010. Clasmogamous flowers were observed in the male sterile lines. In such flowers anthers did not form di-adlephous bundle. Cytological analysis revealed that meiosis proceeded normally till the tetrad stage in those anthers with pollen grains. After which many of the pollen grains turned sterile. In the anthers with pollen grains, dehiscence was not observed, thus creating functional sterility. In many other anthers, pollen mother cells (PMCs) were not formed at all, giving rise to sepalous anthers. In conclusion two mechanisms of male sterility existed, one was premeiotic, where PMCs did not form and in the second, although PMCs gave rise to pollen grains, they were either partially or totally sterile accompanied by nondehiscence of anther wall. Kluwer 2011 Article PeerReviewed application/pdf en http://oar.icrisat.org/132/1/Euphytica182_65-71_2011.pdf Mallikarjuna, N and Jadhav, D R and Srikant, S and Saxena, K B (2011) Cajanus platycarpus (Benth.) Maesen as the donor of new pigeonpea cytoplasmic male sterile (CMS) system. Euphytica, 182 (1). pp. 65-71. http://dx.doi.org/10.1007/s10681-011-0488-9 info:eu-repo/semantics/objectFile info:eu-repo/semantics/humanStartPage