eprintid: 12079
rev_number: 14
eprint_status: archive
userid: 167
dir: disk0/00/01/20/79
datestamp: 2023-05-05 04:31:40
lastmod: 2023-05-05 04:31:40
status_changed: 2023-05-05 04:31:40
type: article
metadata_visibility: show
contact_email: nagaraju.thudumu@icrisat.org
creators_name: Mir, A H
creators_name: Bhat, M A
creators_name: Fayaz, H
creators_name: Wani, A A
creators_name: Dar, S A
creators_name: Maqbool, S
creators_name: Yasin, M
creators_name: Mir, J I
creators_name: Khan, M A
creators_name: Sofi, P A
creators_name: El‑Sappah, A H
creators_name: Thudi, M
creators_name: Varshney, R K
creators_name: Mir, R R
creators_gender: Male
creators_gender: Male
creators_gender: Male
icrisatcreators_name: Thudi, M
icrisatcreators_name: Varshney, R K
affiliation: SKUAST-Kashmir (Sopore)
affiliation: University of Kashmir (Srinagar)
affiliation: SKUAST-Kashmir (Budgam)
affiliation: SKUAST-Kashmir (Shuhama)
affiliation: Rafi Amhad Kidwai (RAK) College of Agriculture, Rajmata Vijayaraje Scindia Krishi Vishwa Vidyalaya (RVSKV) (Sehore)
affiliation: ICAR-Central Institute of Temperate Horticulture (CITH) (Srinagar)
affiliation: School of Agriculture, Forestry and Food Engineering, Yibin University (Yibin)
affiliation: ICRISAT(Patancheru)
affiliation: Department of Agricultural Biotechnology and Biotechnology, Rajendra Prasad Central Agricultural University (Pusa)
affiliation: State Agricultural Biotechnology Centre, Crop & Food Innovation Centre, Food Futures Institute, Murdoch University (Murdoch)
country: India
country: China
country: Australia
title: SSR markers in revealing extent of genetic diversity and phylogenetic relationships among chickpea core collection accessions for Western Himalayas
ispublished: pub
subjects: s1.1
subjects: s2.13
divisions: GRP_ACI
full_text_status: public
keywords: Chickpea, Core collection, Genetic diversity, Microsatellites, Cluster analysis
note: The authors are thankful to ICRISAT (Hyderabad), IIPR (Kanpur), NBPGR (New Delhi) for providing the germplasm for the present study.
abstract: Background The exploration of genetic diversity is the key source of germplasm conservation and potential to broaden its genetic base. The globally growing demand for chickpea suggests superior/climate-resilient varieties, which in turn necessitates the germplasm characterization to unravel underlying genetic variation. 
Methodology and results A chickpea core collection comprising of diverse 192 accessions which include cultivated Cicer arietinum, and wild C. reticulatum, C. echinospermum, and C. microphyllum species were investigated to analyze their genetic diversity and relationship, by assaying 33 unlinked simple sequence repeat (SSR) markers. The results amplified a total of 323 alleles (Na), ranging from 2 to 8 with an average of 4.25 alleles per locus. Expected heterozygosity (He) differed from
0.46 to 0.86 with an average of 0.68. Polymorphic information content (PIC) ranged from 0.73 to 0.98 with an average of 0.89. Analysis of molecular variance (AMOVA) showed that most of the variation was among individuals (87%). Cluster analysis resulted in the formation of four distinct clusters. Cluster I represented all cultivated and clusters II, III, and IV comprised a heterogeneous group of cultivated and wild chickpea accessions.
Conclusion We report considerable diversity and greater resolving power of SSR markers for assessing variability and
interrelationship among the chickpea accessions. The chickpea core is expected to be an efficient resource for breeders for broadening the chickpea genetic base and could be useful for selective breeding of desirable traits and in the identification of target genes for genomics-assisted breeding.
date: 2022-08-25
date_type: published
publication: Molecular Biology Reports
volume: 49
publisher: Springer Netherlands
pagerange: 11469-11479
id_number: https://doi.org/10.1007/s11033-022-07858-4
refereed: FALSE
issn: 1573-4978
official_url: https://link.springer.com/article/10.1186/s43170-022-00082-x
related_url_url: https://scholar.google.com/scholar?hl=en&as_sdt=0%2C5&q=%22Genebanks+and+market+participation%3A+evidence+from+groundnut+farmers+in+Malawi%22&btnG=
related_url_type: pub
citation:   Mir, A H and Bhat, M A and Fayaz, H and Wani, A A and Dar, S A and Maqbool, S and Yasin, M and Mir, J I and Khan, M A and Sofi, P A and El‑Sappah, A H and Thudi, M and Varshney, R K and Mir, R R  (2022) SSR markers in revealing extent of genetic diversity and phylogenetic relationships among chickpea core collection accessions for Western Himalayas.  Molecular Biology Reports, 49.  pp. 11469-11479.  ISSN 1573-4978     
document_url: http://oar.icrisat.org/12079/1/Molecular%20Biology%20Reports_49_11469%E2%80%9311479_2022.pdf