TY  - JOUR
AV  - public
UR  - https://doi.org/10.3389/fpls.2022.962667
IS  - 962667
PB  - Frontiers
TI  - Loss-of-function of triacylglycerol lipases are associated with low flour rancidity in pearl millet [Pennisetum glaucum (L.) R. Br.]
A1  - Aher, R R
A1  - Palakolanu, S R
A1  - Bhunia, R K
A1  - Flyckt, K S.
A1  - Shankhapal, A R
A1  - Ojha, R
A1  - Everard, J D
A1  - Wayne, L L
A1  - Ruddy, B M
A1  - Deonovic, B
A1  - Gupta, S K
A1  - Sharma, K K
A1  - Bhatnagar-Mathur, P
N1  - Authors thank Central Instrumentation Facility (CIF)-NABI for providing key instruments used during the study. Thanks to Joe Shambaugh for assistance with workflow development on the Genedata Expressionist software platform. Authors also thank Günther Daum, Graz University of Technology for kindly providing the yeast lipase-mutant strain.
SN  - 1664-462X
VL  - 13
N2  - Pearl millet is an important cereal crop of semi-arid regions since it is
highly nutritious and climate resilient. However, pearl millet is underutilized
commercially due to the rapid onset of hydrolytic rancidity of seed lipids
post-milling. We investigated the underlying biochemical and molecular
mechanisms of rancidity development in the flour from contrasting inbred
lines under accelerated aging conditions. The breakdown of storage lipids
(triacylglycerols; TAG) was accompanied by free fatty acid accumulation over
the time course for all lines. The high rancidity lines had the highest amount
of FFA by day 21, suggesting that TAG lipases may be the cause of rancidity.
Additionally, the high rancidity lines manifested substantial amounts of volatile
aldehyde compounds, which are characteristic products of lipid oxidation.
Lipases with expression in seed post-milling were sequenced from low and
high rancidity lines. Polymorphisms were identified in two TAG lipase genes
(PgTAGLip1 and PgTAGLip2) from the low rancidity line. Expression in a yeast
model system confirmed these mutants were non-functional. We provide
a direct mechanism to alleviate rancidity in pearl millet flour by identifying
mutations in key TAG lipase genes that are associated with low rancidity. These
genetic variations can be exploited through molecular breeding or precision
genome technologies to develop elite pearl millet cultivars with improved
flour shelf life.
SP  - 1
Y1  - 2022/10/04/
EP  - 13
ID  - icrisat12016
KW  - Pearl millet
KW  -  Pennisetum glaucum (L.) R. Br.
KW  -  Millet
KW  -  Milled flour
KW  -  Lipase
KW  - 
triacylglycerol
KW  -  rancidity
KW  -  shelf life
JF  - Frontiers in Plant Science (TSI)
ER  -