TY - JOUR AV - public UR - https://doi.org/10.3390/plants11131748 IS - 1748 PB - MDPI TI - Effects of Plastic Film Mulching on Soil Enzyme Activities and Stoichiometry in Dryland Agroecosystems A1 - Liu, M A1 - Zhao, X A1 - Hossain, M E A1 - Wang, S A1 - Dong, W A1 - Gopalakrishnan, S A1 - Liu, E N1 - We express our sincere gratitude to the Shouyang Key Dry Farming Field Observation and Experimental Station of the Ministry of Agriculture and Rural Affairs for providing the research platform. SN - 2223-7747 VL - 11(13) N2 - Soil extracellular enzymes are pivotal for microbial nutrient cycling in the ecosystem. In order to study the effects of different nitrogen application rates under plastic film mulching on soil extracellular enzyme activities and stoichiometry, five nitrogen application levels (i.e., 0, 90, 150, 225 and 300 kg·hm?2) were set based on two treatments: plastic film mulching (PM) and no film mulching (LD). We measured the soil extracellular enzyme activities (EEAs) and stoichiometry (EES) of four en-zymes (i.e., ?-1,4-glucosidase (?G), leucine aminopeptidase (LAP), ?-1,4-N-acetylaminoglucosidase (NAG) and alkaline phosphatase (AP)) involved in the C, N and P cycles of soil microorganisms in surface soil at five maize growth stages (seedling stage, jointing stage, trumpet stage, grout stage and harvest stage). The results showed that there were significant differences in soil EEA at different maize growth stages. The soil nutrient content and soil EEA were significantly improved under PM, and the stoichiometric ratio of extracellular enzymes (EC:N:P) was closer to 1:1:1, which indicated that PM was beneficial to the balance of soil nutrients and the activity of microorganisms. At each stage, with the increase in nitrogen application levels, the soil EEA showed a trend of increasing first and then decreasing (or remained unchanged), and both LD and PM treatments reached their highest activity at the 225 kg·hm?2 nitrogen application rate. When the nitrogen application level was less than 225 kg·hm?2, the soil enzyme activity was mainly limited by the N nutrient, and when the nitrogen application level reached 300 kg·hm?2, it was mainly limited by the P nutrient. RDA and correlation analysis showed that the soil C:P, C:N, N:P and pH had significant effects on soil ?G, NAG + LAP and AP activities as well as EC:N, EC:P and EN:P. SP - 1 Y1 - 2022/06/22/ EP - 14 ID - icrisat12006 KW - Nitrogen levels KW - Maize of stage KW - Enzyme activity KW - Enzyme stoichiometric ratio KW - Nutrient limitation JF - Plants (TSI) ER -