"11962","12","archive","3170",,,"disk0/00/01/19/62","2022-03-10 09:07:55","2022-03-10 09:08:14","2022-03-10 09:07:55","article",,,"show",,,,"","","","","","","","","","",,,,"Reddy","P S","","","","","",,"Reddy","P S","","","","",,,,,"","",,,,,"","","ICRISAT (Patancheru)","India","Pearl Millet Aquaporin Gene PgPIP2;6 Improves Abiotic Stress Tolerance in Transgenic Tobacco","pub","ABio","GRP_ACI","CG1","public",,,"Canopy temperature depression (CTD), Exudation rate, Pennisetum glaucum, PgPIP2;6, Progressive drought stress, Transpiration rate, Transpiration efficiency, Vapor pressure deficit",,"We thank Rajeev Varshney and Prasad Bajaj, ICRISAT,
for the basic sequence extraction information from the
pearl millet genome.","Pearl millet [Pennisetum glaucum (L) R. Br.] is an important cereal crop of the semiarid
tropics, which can withstand prolonged drought and heat stress. Considering an active
involvement of the aquaporin (AQP) genes in water transport and desiccation tolerance
besides several basic functions, their potential role in abiotic stress tolerance was
systematically characterized and functionally validated. A total of 34 AQP genes from
P. glaucum were identified and categorized into four subfamilies, viz., plasma membrane
intrinsic proteins (PIPs), tonoplast intrinsic proteins (TIPs), nodulin-26-like intrinsic
proteins (NIPs), and small basic intrinsic proteins (SIPs). Sequence analysis revealed
that PgAQPs have conserved characters of AQP genes with a closer relationship
to sorghum. The PgAQPs were expressed differentially under high vapor pressure
deficit (VPD) and progressive drought stresses where the PgPIP2;6 gene showed
significant expression under high VPD and drought stress. Transgenic tobacco plants
were developed by heterologous expression of the PgPIP2;6 gene and functionally
characterized under different abiotic stresses to further unravel their role. Transgenic
tobacco plants in the T2 generations displayed restricted transpiration and low root
exudation rates in low- and high-VPD conditions. Under progressive drought stress,
wild-type (WT) plants showed a quick or faster decline of soil moisture than transgenics.
While under heat stress, PgPIP2;6 transgenics showed better adaptation to heat
(40C) with high canopy temperature depression (CTD) and low transpiration; under
low-temperature stress, they displayed lower transpiration than their non-transgenic
counterparts. Cumulatively, lower transpiration rate (Tr), low root exudation rate, declined
transpiration, elevated CTD, and lower transpiration indicate that PgPIP2;6 plays a role
under abiotic stress tolerance. Since the PgPIP2;6 transgenic plants exhibited better
adaptation against major abiotic stresses such as drought, high VPD, heat, and cold
stresses by virtue of enhanced transpiration efficiency, it has the potential to engineer
abiotic stress tolerance for sustained growth and productivity of crops.","2022-03","published",,"Frontiers in Plant Science (TSI)","13",,"Frontier Media",,"1-19",,,,,,"doi:10.3389/fpls.2022.820996",,,,,"TRUE",,"1664-462X",,,,,,"","https://doi.org/10.3389/fpls.2022.820996","","",,"","",,,,,,"",,,,,,,"",,,,,"",,,,,"","",,,,,"","",,,,,
"11962",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,"Dhaware","M G","","",,,,,"Dhaware","M G","","",,,,,,,,,,,,,,,"Department of Biochemistry ICMR – National Institute of Nutrition, Hyderabad",,,,"S1.5.1",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
"11962",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,"Sivasakthi","K","","",,,,,"Sivasakthi","K","","",,,,,,,,,,,,,,,"Department of Biotechnology, Vignan’s Foundation for Science, Technology & Research (Deemed to be University), Vadlamudi",,,,"s1000",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
"11962",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,"Divya","K","","",,,,"Female","Divya","K","","",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
"11962",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,"Nagaraju","M","","",,,,,"Sri Cindhuri","K","","",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
"11962",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,"Sri Cindhuri","K","","",,,,"Female","Bhatnagar-Mathur","P","","",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
"11962",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,"Kavi Kishor","P B","","",,,,,"Vadez","V","","",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
"11962",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,"Bhatnagar-Mathur","P","","",,,,"Female","Sharma","K K","","",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
"11962",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,"Vadez","V","","",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
"11962",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,"Sharma","K K","","",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,