Association of Grain Iron and Zinc Content With Other Nutrients in Pearl Millet Germplasm, Breeding Lines, and HybridsMGovindarajauthorAKanattiauthorK NRaiauthorW HPfeifferauthorHShivadeauthorMicronutrient deficiency is most prevalent in developing regions of the world, including Africa and Southeast Asia where pearl millet (Pennisetum glaucum L.) is a major crop. Increasing essential minerals in pearl millet through biofortification could reduce malnutrition caused by deficiency. This study evaluated the extent of variability of micronutrients (Fe, Zn, Mn, and Na) and macronutrients (P, K, Ca, and Mg) and their relationship with Fe and Zn content in 14 trials involving pearl millet hybrids, inbreds, and germplasm. Significant genetic variability ofmacronutrients andmicronutrients was found within and across the trials (Ca: 4.2–40.0mg 100 g−1, Fe: 24–145mg kg−1, Zn: 22– 96mg kg−1, and Na: 3.0–63mg kg−1). Parental lines showed significantly larger variation for nutrients than hybrids, indicating their potential for use in hybrid parent improvement through recurrent selection. Fe and Zn contents were positively correlated and highly significant (r=0.58–0.81; p<0.01). Fe and Zn were positively and significantly correlated with Ca (r = 0.26–0.61; p < 0.05) and Mn (r = 0.24–0.50; p < 0.05). The findings indicate that joint selection for Fe, Zn, and Ca will be effective. Substantial genetic variation and high heritability (>0.60) for multiple grain minerals provide good selection accuracy prospects for genetic enhancement. A highly positive significant correlation between Fe and Zn and the nonsignificant correlation of grain macronutrients and micronutrients with Fe and Zn suggest that there is scope to achieve higher levels of Fe/Zn simultaneously in current pearl millet biofortification efforts without affecting other grain nutrients. Results suggest major prospects for improving multiple nutrients in pearl millet.Plant NutritionBiofortificationFood and Nutrition2022-02Frontiers MediaArticle