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	dc:title "HTML Summary of #11912 \n\nFunctional characterization of the promoter of pearl millet heat shock protein 10 (PgHsp10) in response to abiotic stresses in transgenic tobacco plants\n\n";
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	bibo:abstract "In the present study, the promoter region of the pearlmillet heat shock protein 10 (PgHsp10) genewas cloned and\r\ncharacterized. The PgHsp10 promoter (PgHsp10pro) sequence region has all the cis-motifs required for tissue and\r\nabiotic stress inducibility. The complete PgHsp10pro (PgHsp10PC) region and a series of 5′ truncations of PgHsp10\r\n(PgHsp10D1 and PgHsp10D2) and an antisense formof PgHsp10pro (PgHsp10AS)were cloned into a plant expression\r\nvector (pMDC164) through gateway cloning. All four constructs were separately transformed into tobacco\r\nthrough Agrobacterium-mediated genetic transformation, and PCR-confirmed transgenic plants progressed to\r\nT1 and T2 generations. The T2 transgenic tobacco plants comprising all PgHsp10pro fragments were used for\r\nGUS histochemical and qRT-PCR assays in different tissues under control and abiotic stresses. The PgHsp10PC\r\npro expression was specific to stemand seedlings under control conditions. Under different abiotic stresses, particularly\r\nheat stress, PgHsp10PCpro had relatively higher activity than PgHsp10D1pro, PgHsp10D2pro and\r\nPgHsp10ASpro. PgHsp10pro from a stress resilient crop like pearl millet responds positively to a range of abiotic\r\nstresses, in particular heat, when expressed in heterologous plant systems such as tobacco. Hence, PgHsp10pro\r\nappears to be a potential promoter candidate for developing heat and drought stress-tolerant crop plants."^^xsd:string;
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	dct:subject <http://oar.icrisat.org/id/subject/ABio>,
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	dct:title "Functional characterization of the promoter of pearl millet heat shock protein 10 (PgHsp10) in response to abiotic stresses in transgenic tobacco plants"^^xsd:string;
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