eprintid: 11766
rev_number: 8
eprint_status: archive
userid: 3170
dir: disk0/00/01/17/66
datestamp: 2021-03-26 07:36:29
lastmod: 2021-03-26 07:36:29
status_changed: 2021-03-26 07:36:29
type: article
metadata_visibility: show
creators_name: Kummari, D
creators_name: Palakolanu, S R
creators_name: Kishor, P B K
creators_name: Bhatnagar-Mathur, P
creators_name: Singam, P
creators_name: Vadez, V
creators_name: Sharma, K K
creators_gender: Female
creators_gender: Female
icrisatcreators_name: Palakolanu, S R
icrisatcreators_name: Bhatnagar-Mathur, P
icrisatcreators_name: Vadez, V
icrisatcreators_name: Sharma, K K
affiliation: ICRISAT (Patancheru)
affiliation: Department of Genetics, Osmania University, Hyderabad
affiliation: Department of Biotechnology, Vignan’s Foundation for Science, Technology and Research Deemed to be University, Vadlamudi
country: India
title: An update and perspectives on the use of promoters in plant genetic engineering
ispublished: pub
subjects: G1
divisions: CRPS3
crps: CG1
full_text_status: public
keywords: CaMV35S promoter, Constitutive promoters, Synthetic promoters, Genetic engineering, RNA pol
II promoters, RNA pol III promoters, U3 promoter, U6 promoter
note: PSR acknowledges financial support from the Department
of Science and Technology, Govt. of India, New
Delhi, for a fellowship and research grant through the
INSPIRE Faculty Award No. IFALSPA-06 and Young
Scientist Award SB/YS/LS-12/2013. This work was
undertaken as part of the CGIAR Research Program on
Grain Legumes & Dryland Cereals (CRP-GLDC).
abstract: Genetically engineered plants have varied applications in agriculture for enhancing the values of food and feed.
Genetic engineering aims to introduce selected genetic regions with desirable traits into target plants for both
spatial and temporal expressions. Promoters are the key elements responsible for regulating gene expressions
by modulating the transcription factors (TFs) through recognition of RNA polymerases. Based on their
recognition and expression, RNA polymerases were categorized into RNA pol II and pol III promoters.
Promoter activity and specificity are the two prime parameters in regulating the transgene expression. Since the
use of constitutive promoters like Cauliflower mosaic virus (CaMV) 35S may lead to adverse effects on nontarget
organisms or ecosystem, inducible/tissue specific promoters and/or the RNA pol III promoters provide
myriad opportunities for gene expressions with controlled regulation and with minimum adverse effects.
Besides their role in transgene expression, their influence in synthetic biology and genome editing are also
discussed. This review provides an update on the importance, current prospects, and insight into the advantages
and disadvantages of promoters reported thus far would help to utilize them in the endeavour to develop
nutritionally and agronomically improved transgenic crops for commercialization.
date: 2020-09
date_type: published
publication: Journal of Biosciences (TSI)
volume: 45
number: 1
publisher: Springer Verlag
pagerange: 1-24
id_number: doi:10.1007/s12038-020-00087-6
refereed: TRUE
issn: 0250-5991
official_url: https://doi.org/10.1007/s12038-020-00087-6
related_url_url: https://scholar.google.com/scholar?hl=en&as_sdt=0%2C5&q=10.1007%2Fs12038-020-00087-6&btnG=
related_url_type: pub
citation:   Kummari, D and Palakolanu, S R and Kishor, P B K and Bhatnagar-Mathur, P and Singam, P and Vadez, V and Sharma, K K  (2020) An update and perspectives on the use of promoters in plant genetic engineering.  Journal of Biosciences (TSI), 45 (1).  pp. 1-24.  ISSN 0250-5991     
document_url: http://oar.icrisat.org/11766/1/s12038-020-00087-6.pdf