TY  - JOUR
AV  - public
A1  - Kummari, D
A1  - Palakolanu, S R
A1  - Kishor, P B K
A1  - Bhatnagar-Mathur, P
A1  - Singam, P
A1  - Vadez, V
A1  - Sharma, K K
TI  - An update and perspectives on the use of promoters in plant genetic engineering
UR  - https://doi.org/10.1007/s12038-020-00087-6
JF  - Journal of Biosciences (TSI)
SN  - 0250-5991
PB  - Springer Verlag
N1  - PSR acknowledges financial support from the Department
of Science and Technology, Govt. of India, New
Delhi, for a fellowship and research grant through the
INSPIRE Faculty Award No. IFALSPA-06 and Young
Scientist Award SB/YS/LS-12/2013. This work was
undertaken as part of the CGIAR Research Program on
Grain Legumes & Dryland Cereals (CRP-GLDC).
N2  - Genetically engineered plants have varied applications in agriculture for enhancing the values of food and feed.
Genetic engineering aims to introduce selected genetic regions with desirable traits into target plants for both
spatial and temporal expressions. Promoters are the key elements responsible for regulating gene expressions
by modulating the transcription factors (TFs) through recognition of RNA polymerases. Based on their
recognition and expression, RNA polymerases were categorized into RNA pol II and pol III promoters.
Promoter activity and specificity are the two prime parameters in regulating the transgene expression. Since the
use of constitutive promoters like Cauliflower mosaic virus (CaMV) 35S may lead to adverse effects on nontarget
organisms or ecosystem, inducible/tissue specific promoters and/or the RNA pol III promoters provide
myriad opportunities for gene expressions with controlled regulation and with minimum adverse effects.
Besides their role in transgene expression, their influence in synthetic biology and genome editing are also
discussed. This review provides an update on the importance, current prospects, and insight into the advantages
and disadvantages of promoters reported thus far would help to utilize them in the endeavour to develop
nutritionally and agronomically improved transgenic crops for commercialization.
KW  - CaMV35S promoter
KW  -  Constitutive promoters
KW  -  Synthetic promoters
KW  -  Genetic engineering
KW  -  RNA pol
II promoters
KW  -  RNA pol III promoters
KW  -  U3 promoter
KW  -  U6 promoter
Y1  - 2020/09//
SP  - 1
ID  - icrisat11766
EP  - 24
VL  - 45
IS  - 1
ER  -