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	dc:format "text/html";
	dc:title "HTML Summary of #11755 \n\nSurvey sequencing and in-silico development and validation of genomic SSR markers in Indian dill seed\n\n";
	foaf:primaryTopic <http://oar.icrisat.org/id/eprint/11755> .

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<http://oar.icrisat.org/id/eprint/11755>
	bibo:abstract "Indian dill seed (Anethum sowa Roxb. ex Fleming) is an important member of family Apiaceae. It is a significant\r\nseed spice having many medicinal properties. But, this aromatic herb is still orphan from breeding\r\nand crop improvement perspective as no serious attention has been given by breeders. To swift the\r\nbreeding program, molecular markers play the main role. But due to the paucity of markers, breeding is\r\nslower in dill seed. Therefore, an attempt was made to develop the genomic SSR markers in dill seed\r\nthrough next-generation sequencing. A total of 2119.51 Mbp of raw data were generated on the Miseq\r\nNGS platform during survey sequencing. In 2,25,956 contigs created by Spades assembler, a total\r\n48,951 repeat motifs were identified. A set of 20,294 primer pairs (dimer to hexamer repeats) were produced.\r\nAmong detected repeat motifs, 48.89% was mononucleotides. Of 12 primers, 10 (83%) primers\r\ncould be successfully amplified in dill seed and produced 11 amplicons. During cross-genera amplification\r\nof markers, 9 out of 10 primers could be successfully amplified in related genera. Developed markers\r\ncan also be used to initiate the molecular breeding program, association mapping and to assess the evolutionary\r\nrelationship among seed spices."^^xsd:string;
	bibo:authorList <http://oar.icrisat.org/id/eprint/11755#authors>;
	bibo:issue "1";
	bibo:status <http://purl.org/ontology/bibo/status/peerReviewed>,
		<http://purl.org/ontology/bibo/status/published>;
	bibo:volume "32";
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	dct:creator <http://oar.icrisat.org/id/person/ext-2bd4b27c4a4717f0dcc3f0401ef4134a>,
		<http://oar.icrisat.org/id/person/ext-554e2708fee55191dda8e0f5ed64e322>,
		<http://oar.icrisat.org/id/person/ext-c8f145a03a47fa6fbb2bc9ca20639c81>,
		<http://oar.icrisat.org/id/person/ext-cf5659b4ec20d684bd125a97df5b38d1>;
	dct:date "2020-01";
	dct:isPartOf <http://oar.icrisat.org/id/repository>,
		<http://oar.icrisat.org/id/publication/ext-10183647>;
	dct:publisher <http://oar.icrisat.org/id/org/ext-f308aad1ef8f70546c3a197f104f2ad5>;
	dct:subject <http://oar.icrisat.org/id/subject/s2.12>,
		<http://oar.icrisat.org/id/subject/s2342>;
	dct:title "Survey sequencing and in-silico development and validation of genomic SSR markers in Indian dill seed"^^xsd:string;
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		ep:ArticleEPrint,
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