"11755","8","archive","3170",,,"disk0/00/01/17/55","2021-03-22 09:10:57","2021-03-22 09:10:57","2021-03-22 09:10:57","article",,,"show",,,,"","","","","","","","","","",,,,"Kumar","S","","","","","","","Gandham","P","","","","",,,,,"","",,,,,"","","Department of Agricultural Biotechnology, Anand Agricultural University, Anand, India","","Survey sequencing and in-silico development and validation of genomic SSR markers in Indian dill seed","pub","s2.12","S15","","public",,,"Anethum, Seed spices, SSR, Transferability",,"Authors acknowledge Anand Agricultural University, India for
providing all necessary facilities. SK acknowledges the INSA, N.
Delhi for providing visiting scientist fellowship at ICRISAT, Hyderabad
during the analysis of data.","Indian dill seed (Anethum sowa Roxb. ex Fleming) is an important member of family Apiaceae. It is a significant
seed spice having many medicinal properties. But, this aromatic herb is still orphan from breeding
and crop improvement perspective as no serious attention has been given by breeders. To swift the
breeding program, molecular markers play the main role. But due to the paucity of markers, breeding is
slower in dill seed. Therefore, an attempt was made to develop the genomic SSR markers in dill seed
through next-generation sequencing. A total of 2119.51 Mbp of raw data were generated on the Miseq
NGS platform during survey sequencing. In 2,25,956 contigs created by Spades assembler, a total
48,951 repeat motifs were identified. A set of 20,294 primer pairs (dimer to hexamer repeats) were produced.
Among detected repeat motifs, 48.89% was mononucleotides. Of 12 primers, 10 (83%) primers
could be successfully amplified in dill seed and produced 11 amplicons. During cross-genera amplification
of markers, 9 out of 10 primers could be successfully amplified in related genera. Developed markers
can also be used to initiate the molecular breeding program, association mapping and to assess the evolutionary
relationship among seed spices.","2020-01","published",,"Journal of King Saud University - Science (TSI)","32","1","Elsevier",,"862-866",,,,,,"doi:10.1016/j.jksus.2019.04.006",,,,,"TRUE",,"1018-3647",,,,,,"","https://doi.org/10.1016/j.jksus.2019.04.006","https://scholar.google.com/scholar?hl=en&as_sdt=0%2C5&q=10.1016%2Fj.jksus.2019.04.006&btnG=","pub",,"","",,,,,,"",,,,,,,"",,,,,"",,,,,"","",,,,,"","",,,,,
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