<mods:mods version="3.3" xsi:schemaLocation="http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-3.xsd" xmlns:mods="http://www.loc.gov/mods/v3" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance"><mods:titleInfo><mods:title>Genome-Wide DArTSeq Genotyping and Phenotypic Based Assessment of Within and Among Accessions Diversity and Effective Sample Size in the Diverse Sorghum, Pearl Millet, and Pigeonpea Landraces</mods:title></mods:titleInfo><mods:name type="personal"><mods:namePart type="given">V</mods:namePart><mods:namePart type="family">Allan</mods:namePart><mods:role><mods:roleTerm type="text">author</mods:roleTerm></mods:role></mods:name><mods:name type="personal"><mods:namePart type="given">M</mods:namePart><mods:namePart type="family">Vetriventhan</mods:namePart><mods:role><mods:roleTerm type="text">author</mods:roleTerm></mods:role></mods:name><mods:name type="personal"><mods:namePart type="given">R</mods:namePart><mods:namePart type="family">Senthil</mods:namePart><mods:role><mods:roleTerm type="text">author</mods:roleTerm></mods:role></mods:name><mods:name type="personal"><mods:namePart type="given">S</mods:namePart><mods:namePart type="family">Geetha</mods:namePart><mods:role><mods:roleTerm type="text">author</mods:roleTerm></mods:role></mods:name><mods:name type="personal"><mods:namePart type="given">S</mods:namePart><mods:namePart type="family">Deshpande</mods:namePart><mods:role><mods:roleTerm type="text">author</mods:roleTerm></mods:role></mods:name><mods:name type="personal"><mods:namePart type="given">A</mods:namePart><mods:namePart type="family">Rathore</mods:namePart><mods:role><mods:roleTerm type="text">author</mods:roleTerm></mods:role></mods:name><mods:name type="personal"><mods:namePart type="given">V</mods:namePart><mods:namePart type="family">Kumar</mods:namePart><mods:role><mods:roleTerm type="text">author</mods:roleTerm></mods:role></mods:name><mods:name type="personal"><mods:namePart type="given">P</mods:namePart><mods:namePart type="family">Singh</mods:namePart><mods:role><mods:roleTerm type="text">author</mods:roleTerm></mods:role></mods:name><mods:name type="personal"><mods:namePart type="given">S</mods:namePart><mods:namePart type="family">Reddymalla</mods:namePart><mods:role><mods:roleTerm type="text">author</mods:roleTerm></mods:role></mods:name><mods:name type="personal"><mods:namePart type="given">V C R</mods:namePart><mods:namePart type="family">Azevedo</mods:namePart><mods:role><mods:roleTerm type="text">author</mods:roleTerm></mods:role></mods:name><mods:abstract>Germplasm should be conserved in such a way that the genetic integrity of a given&#13;
accession is maintained. In most genebanks, landraces constitute a major portion of&#13;
collections, wherein the extent of genetic diversity within and among landraces of crops&#13;
vary depending on the extent of outcrossing and selection intensity infused by farmers.&#13;
In this study, we assessed the level of diversity within and among 108 diverse landraces&#13;
and wild accessions using both phenotypic and genotypic characterization. This included&#13;
36 accessions in each of sorghum, pearl millet, and pigeonpea, conserved at ICRISAT&#13;
genebank. We genotyped about 15 to 25 individuals within each accession, totaling&#13;
1,980 individuals using the DArTSeq approach. This resulted in 45,249, 19,052, and&#13;
8,211 high-quality single nucleotide polymorphisms (SNPs) in pearl millet, sorghum,&#13;
and pigeonpea, respectively. Sorghum had the lowest average phenotypic (0.090) and&#13;
genotypic (0.135) within accession distances, while pearl millet had the highest average&#13;
phenotypic (0.227) and genotypic (0.245) distances. Pigeonpea had an average of&#13;
0.203 phenotypic and 0.168 genotypic within accession distances. Analysis of molecular&#13;
variance also confirms the lowest variability within accessions of sorghum (26.3%)&#13;
and the highest of 80.2% in pearl millet, while an intermediate in pigeonpea (57.0%).&#13;
The effective sample size required to capture maximum variability and to retain rare&#13;
alleles while regeneration ranged from 47 to 101 for sorghum, 155 to 203 for pearl&#13;
millet, and 77 to 89 for pigeonpea accessions. This study will support genebank&#13;
curators, in understanding the dynamics of population within and among accessions,&#13;
in devising appropriate germplasm conservation strategies, and aid in their utilization for&#13;
crop improvement.</mods:abstract><mods:classification authority="lcc">Pearl Millet</mods:classification><mods:classification authority="lcc">Pigeonpea</mods:classification><mods:classification authority="lcc">Sorghum</mods:classification><mods:classification authority="lcc">Genetics and Genomics</mods:classification><mods:originInfo><mods:dateIssued encoding="iso8061">2020-12</mods:dateIssued></mods:originInfo><mods:originInfo><mods:publisher>Frontiers Media</mods:publisher></mods:originInfo><mods:genre>Article</mods:genre></mods:mods>