eprintid: 11725
rev_number: 11
eprint_status: archive
userid: 3170
dir: disk0/00/01/17/25
datestamp: 2021-03-07 07:07:02
lastmod: 2021-03-07 07:07:02
status_changed: 2021-03-07 07:07:02
type: article
metadata_visibility: show
creators_name: Biswas, K
creators_name: Tarafdar, A
creators_name: Kumar, R
creators_name: Singhvi, N
creators_name: Ghosh, P
creators_name: Sharma, M
creators_name: Pabbi, S
creators_name: Shukla, P
icrisatcreators_name: Tarafdar, A
icrisatcreators_name: Sharma, M
affiliation: Department of Biotechnology, Visva Bharati University, Santiniketan, India
affiliation: ICRISAT (Patancheru)
affiliation: Department of Human Genetics and Molecular Medicine, Central University of Punjab, Bathinda
affiliation: Department of Zoology, University of Delhi, New Delhi
affiliation: Department of Botany, University of Kalyani, Kalyani
affiliation: Centre for Conservation and Utilisation of Blue Green Algae, Division of Microbiology, Indian Agricultural Research Institute, New Delhi, India
affiliation: Enzyme Technology and Protein Bioinformatics Laboratory, Department of Microbiology, Maharshi Dayanand University, Rohtak
country: India
title: Molecular Analysis of Disease-Responsive Genes Revealing the Resistance Potential Against Fusarium Wilt (Fusarium udum Butler) Dependent on Genotype Variability in the Leguminous Crop Pigeonpea
ispublished: pub
subjects: s1.2
subjects: s2.15
subjects: s35
divisions: CRPS2
full_text_status: public
keywords: Disease incidence, Fusarium udum, Gene expression, Innate resistance, Pathogenesis, Pigeonpea
note: PS acknowledges the infrastructural support from Department
of Science and Technology, New Delhi, Govt. of India, through
FIST grant (Grant No. 1196 SR/FST/LS-I/2017/4). The authors
wish to acknowledge Dr. Vipin Gupta, PhiXgen Pvt. Ltd.,
India for helping in in-silico experimental design in the
study. The authors are also thankful to Legumes Pathology,
ICRISAT, Hyderabad, India for providing biological samples
to conduct the study.
abstract: Fusarium wilt (FW), caused by Fusarium udum Butler (FU), is among the
challenging factors in the production of pigeonpea. Therefore, exploring a superior
pigeonpea genotype from landraces or local cultivars through the selection of
innate resistance to FW using different biological and molecular approaches, and
validating its resistance response, could be an alternative to sustainable crop
improvement. Five distinct pigeonpea genotypes, with resistant (ICP2894) and
susceptible (ICP2376) controls, were selected on the basis of the incidence percentage
of FW, from three different states of India. Among them, the cultivar Richa,
which displayed low incidence of FW (10.0%) during the genotype evaluation,
was further examined for its innate resistance to FW. Molecular characterization
of antioxidant (AO) enzyme [APX and SOD] and pathogenesis-related (PR) protein
[CHS and b-1, 3-glucanase] families were performed. The obtained results of
reverse transcription-polymerase chain reaction-based expression study and in silico
analysis showed a higher level of induction of PR and AO genes, and the
strong interaction of their putative proteins with fungal cellobiohydrolase-c protein
established their antifungal activity, conferring early plant defense responses to FU in Richa. Our study demonstrated a strong and combinatorial approach
involving biological assay, molecular experiments, and in silico analysis to identify
a superior pigeonpea genotype that was resistant to FW across a major
biogeographic region.
date: 2020-08
date_type: published
publication: Frontiers in Genetics (TSI)
volume: 11
publisher: FRONTIERS MEDIA SA
pagerange: 1-15
id_number: doi:10.3389/fgene.2020.00862
refereed: TRUE
issn: 1664-8021
official_url: https://doi.org/10.3389/fgene.2020.00862
related_url_url: https://scholar.google.com/scholar?hl=en&as_sdt=0%2C5&q=10.3389%2Ffgene.2020.00862&btnG=
related_url_type: pub
funders: UGC
citation:   Biswas, K and Tarafdar, A and Kumar, R and Singhvi, N and Ghosh, P and Sharma, M and Pabbi, S and Shukla, P  (2020) Molecular Analysis of Disease-Responsive Genes Revealing the Resistance Potential Against Fusarium Wilt (Fusarium udum Butler) Dependent on Genotype Variability in the Leguminous Crop Pigeonpea.  Frontiers in Genetics (TSI), 11.  pp. 1-15.  ISSN 1664-8021     
document_url: http://oar.icrisat.org/11725/1/fgene-11-00862.pdf