TY  - JOUR
AV  - public
A1  - Biswas, K
A1  - Tarafdar, A
A1  - Kumar, R
A1  - Singhvi, N
A1  - Ghosh, P
A1  - Sharma, M
A1  - Pabbi, S
A1  - Shukla, P
TI  - Molecular Analysis of Disease-Responsive Genes Revealing the Resistance Potential Against Fusarium Wilt (Fusarium udum Butler) Dependent on Genotype Variability in the Leguminous Crop Pigeonpea
UR  - https://doi.org/10.3389/fgene.2020.00862
JF  - Frontiers in Genetics (TSI)
SN  - 1664-8021
PB  - FRONTIERS MEDIA SA
N1  - PS acknowledges the infrastructural support from Department
of Science and Technology, New Delhi, Govt. of India, through
FIST grant (Grant No. 1196 SR/FST/LS-I/2017/4). The authors
wish to acknowledge Dr. Vipin Gupta, PhiXgen Pvt. Ltd.,
India for helping in in-silico experimental design in the
study. The authors are also thankful to Legumes Pathology,
ICRISAT, Hyderabad, India for providing biological samples
to conduct the study.
N2  - Fusarium wilt (FW), caused by Fusarium udum Butler (FU), is among the
challenging factors in the production of pigeonpea. Therefore, exploring a superior
pigeonpea genotype from landraces or local cultivars through the selection of
innate resistance to FW using different biological and molecular approaches, and
validating its resistance response, could be an alternative to sustainable crop
improvement. Five distinct pigeonpea genotypes, with resistant (ICP2894) and
susceptible (ICP2376) controls, were selected on the basis of the incidence percentage
of FW, from three different states of India. Among them, the cultivar Richa,
which displayed low incidence of FW (10.0%) during the genotype evaluation,
was further examined for its innate resistance to FW. Molecular characterization
of antioxidant (AO) enzyme [APX and SOD] and pathogenesis-related (PR) protein
[CHS and b-1, 3-glucanase] families were performed. The obtained results of
reverse transcription-polymerase chain reaction-based expression study and in silico
analysis showed a higher level of induction of PR and AO genes, and the
strong interaction of their putative proteins with fungal cellobiohydrolase-c protein
established their antifungal activity, conferring early plant defense responses to FU in Richa. Our study demonstrated a strong and combinatorial approach
involving biological assay, molecular experiments, and in silico analysis to identify
a superior pigeonpea genotype that was resistant to FW across a major
biogeographic region.
KW  - Disease incidence
KW  -  Fusarium udum
KW  -  Gene expression
KW  -  Innate resistance
KW  -  Pathogenesis
KW  -  Pigeonpea
Y1  - 2020/08//
SP  - 1
ID  - icrisat11725
EP  - 15
VL  - 11
ER  -