%0 Journal Article
%@ 1664-8021
%A Biswas, K
%A Tarafdar, A
%A Kumar, R
%A Singhvi, N
%A Ghosh, P
%A Sharma, M
%A Pabbi, S
%A Shukla, P
%D 2020
%F icrisat:11725
%I FRONTIERS MEDIA SA
%J Frontiers in Genetics (TSI)
%K Disease incidence, Fusarium udum, Gene expression, Innate resistance, Pathogenesis, Pigeonpea
%P 1-15
%T Molecular Analysis of Disease-Responsive Genes Revealing the Resistance Potential Against Fusarium Wilt (Fusarium udum Butler) Dependent on Genotype Variability in the Leguminous Crop Pigeonpea
%U http://oar.icrisat.org/11725/
%V 11
%X Fusarium wilt (FW), caused by Fusarium udum Butler (FU), is among the  challenging factors in the production of pigeonpea. Therefore, exploring a superior  pigeonpea genotype from landraces or local cultivars through the selection of  innate resistance to FW using different biological and molecular approaches, and  validating its resistance response, could be an alternative to sustainable crop  improvement. Five distinct pigeonpea genotypes, with resistant (ICP2894) and  susceptible (ICP2376) controls, were selected on the basis of the incidence percentage  of FW, from three different states of India. Among them, the cultivar Richa,  which displayed low incidence of FW (10.0%) during the genotype evaluation,  was further examined for its innate resistance to FW. Molecular characterization  of antioxidant (AO) enzyme [APX and SOD] and pathogenesis-related (PR) protein  [CHS and b-1, 3-glucanase] families were performed. The obtained results of  reverse transcription-polymerase chain reaction-based expression study and in silico  analysis showed a higher level of induction of PR and AO genes, and the  strong interaction of their putative proteins with fungal cellobiohydrolase-c protein  established their antifungal activity, conferring early plant defense responses to FU in Richa. Our study demonstrated a strong and combinatorial approach  involving biological assay, molecular experiments, and in silico analysis to identify  a superior pigeonpea genotype that was resistant to FW across a major  biogeographic region.
%Z PS acknowledges the infrastructural support from Department  of Science and Technology, New Delhi, Govt. of India, through  FIST grant (Grant No. 1196 SR/FST/LS-I/2017/4). The authors  wish to acknowledge Dr. Vipin Gupta, PhiXgen Pvt. Ltd.,  India for helping in in-silico experimental design in the  study. The authors are also thankful to Legumes Pathology,  ICRISAT, Hyderabad, India for providing biological samples  to conduct the study.