eprintid: 11688
rev_number: 13
eprint_status: archive
userid: 3170
dir: disk0/00/01/16/88
datestamp: 2021-01-06 02:30:20
lastmod: 2021-03-24 03:53:19
status_changed: 2021-01-06 02:30:20
type: article
metadata_visibility: show
creators_name: Shinde, H
creators_name: Dudhate, A
creators_name: Anand, L
creators_name: Tsugama, D
creators_name: Gupta, S K
creators_name: Liu, S
creators_name: Takano, T
creators_gender: Female
icrisatcreators_name: Gupta, S K
affiliation: Asian Natural Environmental Science Center (ANESC), The University of Tokyo, Nishitokyo-shi, Tokyo, Japan
affiliation: Environmental Epigenetics and Genetics Group, Department of Horticulture, College of Agriculture, Food and Environment, University of Kentucky, Lexington, KY
affiliation: ICRISAT (Patancheru)
affiliation: State Key Laboratory of Subtropical Silviculture, Zhejiang A and F University, Lin’an, Hangzhou
country: Japan
country: USA
country: India
country: China
title: Small RNA sequencing reveals the role of pearl millet miRNAs and their targets in salinity stress responses
ispublished: pub
subjects: ABio
subjects: PLB1
subjects: S1.5.1
divisions: CRPS2
crps: CG1
full_text_status: public
keywords: Pearl millet, Salinity stress, miRNAs, Transcription factors, Auxin response pathway
abstract: Pearl millet [Pennisetum glaucum (L.) R. Br.] is an important crop in arid and semi-arid areas of India and
Africa. It is well known for its tolerance to abiotic stresses, but it lags behind other cereals in terms of
research and development. MicroRNAs (miRNAs) are a versatile group of small regulatory RNAs of 20�22
bases that play important roles in plant growth, development, and stress responses. However, the regulatory
mechanisms underlying miRNA-mediated responses to salinity stress in pearl millet are still unclear. In this
study, we performed small RNA sequencing to identify conserved and novel miRNAs from the salinity tolerant
pearl millet genotype. In total, 130 million sequence reads were generated, and 81 conserved and 14
novel miRNAs were identified as salinity stress responsive microRNAs. We also performed target prediction
for these miRNAs, and a total of 448 pearl millet mRNAs were identified as the targets. Among these target
mRNAs, 122 (~25%) encode transcription factors. A pathway analysis showed that differentially expressed
miRNAs and their target genes can regulate the auxin response pathway. Quantitative real-time PCR analysis
of miRNAs and their targets showed consistent expression patterns. These results suggest that miRNAs play
a role in salinity stress tolerance in pearl millet.
date: 2020-06
date_type: published
publication: South African Journal of Botany (TSI)
volume: 132
publisher: Elsevier
pagerange: 395-402
id_number: doi:10.1016/j.sajb.2020.06.011
refereed: TRUE
issn: 0254-6299
official_url: https://doi.org/10.1016/j.sajb.2020.06.011
related_url_url: https://scholar.google.com/scholar?hl=en&as_sdt=0%2C5&q=10.1016%2Fj.sajb.2020.06.011&btnG=
related_url_type: pub
funders: Kakenhi Grant from Japan Society of the Promotion of Science (JSPS)
citation:   Shinde, H and Dudhate, A and Anand, L and Tsugama, D and Gupta, S K and Liu, S and Takano, T  (2020) Small RNA sequencing reveals the role of pearl millet miRNAs and their targets in salinity stress responses.  South African Journal of Botany (TSI), 132.  pp. 395-402.  ISSN 0254-6299     
document_url: http://oar.icrisat.org/11688/1/Shinde%20et%20al%202020.pdf