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	bibo:abstract "Pearl millet [Pennisetum glaucum (L.) R. Br.] is an important crop in arid and semi-arid areas of India and\r\nAfrica. It is well known for its tolerance to abiotic stresses, but it lags behind other cereals in terms of\r\nresearch and development. MicroRNAs (miRNAs) are a versatile group of small regulatory RNAs of 20�22\r\nbases that play important roles in plant growth, development, and stress responses. However, the regulatory\r\nmechanisms underlying miRNA-mediated responses to salinity stress in pearl millet are still unclear. In this\r\nstudy, we performed small RNA sequencing to identify conserved and novel miRNAs from the salinity tolerant\r\npearl millet genotype. In total, 130 million sequence reads were generated, and 81 conserved and 14\r\nnovel miRNAs were identified as salinity stress responsive microRNAs. We also performed target prediction\r\nfor these miRNAs, and a total of 448 pearl millet mRNAs were identified as the targets. Among these target\r\nmRNAs, 122 (~25%) encode transcription factors. A pathway analysis showed that differentially expressed\r\nmiRNAs and their target genes can regulate the auxin response pathway. Quantitative real-time PCR analysis\r\nof miRNAs and their targets showed consistent expression patterns. These results suggest that miRNAs play\r\na role in salinity stress tolerance in pearl millet."^^xsd:string;
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