%O This work was supported by the National Natural Science
Foundation of China (31801856 to X.S, 31371282,
2016YFD0101001 to X.W), the Hebei Province Higher Education
Youth Talents Program (BJ2018016 to X.S), China-Hebei 100
Scholars Supporting Project to X.W. (E2013100003), and the
China Postdoctoral Science Foundation (2020M673188 to X.S).
The genome sequencing, Hi-C sequencing and primary assembly
were performed with the help of Novogene.
%K Celery genome, Paleopolyploidizations, Karyotype Reconstruction, Resistance gene, Coumarins.
%A X Song
%A P Sun
%A J Yuan
%A K Gong
%A N Li
%A F Meng
%A Z Zhang
%A X Li
%A J Hu
%A J Wang
%A Q Yang
%A B Jiao
%A F Nie
%A T Liu
%A W Chen
%A S Feng
%A Q Pei
%A T Yu
%A X Kang
%A W Zhao
%A C Cui
%A Y Yu
%A T Wu
%A L Shan
%A M Liu
%A Z Qin
%A H Lin
%A R K Varshney
%A X Q Li
%A A H Paterson
%A X Wang
%I Association of Applied Biologist
%L icrisat11685
%J Plant Biotechnology Journal (TSI)
%P 1-14
%R doi:10.1111/pbi.13499
%D 2020
%X Celery (Apium graveolens L. 2n = 2x = 22), a member of the Apiaceae family, is among the
most important and globally grown vegetables. Here, we report a high-quality genome sequence
assembly, anchored to 11 chromosomes, with total length of 3.33 Gb and N50 scaffold length
of 289.78 Mb. Most (92.91%) of the genome is composed of repetitive sequences, with
62.12% of 31 326 annotated genes confined to the terminal 20% of chromosomes.
Simultaneous bursts of shared long-terminal repeats (LTRs) in different Apiaceae plants suggest
inter-specific exchanges. Two ancestral polyploidizations were inferred, one shared by Apiales
taxa and the other confined to Apiaceae. We reconstructed 8 Apiales proto-chromosomes,
inferring their evolutionary trajectories from the eudicot common ancestor to extant plants.
Transcriptome sequencing in three tissues (roots, leaves and petioles), and varieties with
different-coloured petioles, revealed 4 and 2 key genes in pathways regulating anthocyanin and
coumarin biosynthesis, respectively. A remarkable paucity of NBS disease-resistant genes in
celery (62) and other Apiales was explained by extensive loss and limited production of these
genes during the last ~10 million years, raising questions about their biotic defence mechanisms
and motivating research into effects of chemicals, for example coumarins, that give off
distinctive odours. Celery genome sequencing and annotation facilitates further research into
important gene functions and breeding, and comparative genomic analyses in Apiales.
%T The celery genome sequence reveals sequential paleo‐polyploidizations, karyotype evolution and resistance gene reduction in apiales