eprintid: 11680
rev_number: 10
eprint_status: archive
userid: 3170
dir: disk0/00/01/16/80
datestamp: 2020-12-29 12:38:47
lastmod: 2020-12-29 12:38:47
status_changed: 2020-12-29 12:38:47
type: article
metadata_visibility: show
creators_name: Gunnabo, A H
creators_name: van Heerwaarden, J
creators_name: Geurts, R
creators_name: Wolde-meskel, E
creators_name: Degefu, T
creators_name: Giller, K E
icrisatcreators_name: Degefu, T
affiliation: Plant Production Systems Group, Wageningen University and  Research, Wageningen, The Netherlands
affiliation: Laboratory of Molecular Biology, Department of Plant Science,  Wageningen University, Wageningen
affiliation: World Agroforestry Centre (ICRAF), Addis Ababa
affiliation: ICRISAT (Ethiopia)
country: The Netherlands
country: Ethiopia
title: Phylogeography and Symbiotic Effectiveness of Rhizobia Nodulating Chickpea (Cicer arietinum L.) in Ethiopia
ispublished: pub
subjects: MB1
subjects: s1.1
subjects: s355
subjects: s54
divisions: CRPS5
full_text_status: public
keywords: Genetic diversity, Genospecies, Haplotypes, Mesorhizobial, strains, Nucleotides, Spatial patterns
note: We thank the Bill & Melinda Gates Foundation for
partnering in this research through a grant to the Wageningen University
to support the project N2Africa: Putting Nitrogen Fixation to Work for
Smallholder Farmers in Africa
abstract: Chickpea (Cicer arietinum L.) used to be considered a restrictive host that nodulated and fixed nitrogen only with Mesorhizobium
ciceri and M.mediterraneum. Recent analysis revealed that chickpea can also establish effective symbioseswith strains of several
other Mesorhizobium species such as M. loti, M. haukuii, M. amorphae, M. muleiense, etc. These strains vary in their nitrogen
fixation potential inviting further exploration. We characterized newly collected mesorhizobial strains isolated from various
locations in Ethiopia to evaluate genetic diversity, biogeographic structure and symbiotic effectiveness. Symbiotic effectiveness
was evaluated in Leonard Jars using a locally released chickpea cultivar “Nattoli”. Most of the new isolates belonged to a clade
related to M. plurifarium, with very few sequence differences, while the total collection of strains contained three additional
mesorhizobial genospecies associated with M. ciceri, M. abyssinicae and an unidentified Mesorhizobium species isolated from a
wild host in Eritrea. The four genospecies identified represented a subset of the eight major Mesorhizobium clades recently
reported for Ethiopia based on metagenomic data. All Ethiopian strains had nearly identical symbiotic genes that grouped them in
a single cluster with M. ciceri, M. mediterraneum and M. muleiense, but not with M. plurifarium. Some phylogeographic
structure was observed, with elevation and geography explaining some of the genetic differences among strains, but the relation
between genetic identity and symbiotic effectiveness was observed to be weak.
date: 2020-10
date_type: published
publication: Microbial Ecology (TSI)
publisher: Springer
id_number: doi:10.1007/s00248-020-01620-8
refereed: TRUE
issn: 0095-3628
official_url: https://doi.org/10.1007/s00248-020-01620-8
related_url_url: https://scholar.google.com/scholar?hl=en&as_sdt=0%2C5&q=10.1007%2Fs00248-020-01620-8&btnG=
related_url_type: pub
citation:   Gunnabo, A H and van Heerwaarden, J and Geurts, R and Wolde-meskel, E and Degefu, T and Giller, K E  (2020) Phylogeography and Symbiotic Effectiveness of Rhizobia Nodulating Chickpea (Cicer arietinum L.) in Ethiopia.  Microbial Ecology (TSI).   ISSN 0095-3628     
document_url: http://oar.icrisat.org/11680/1/Gunnabo2020_Article_PhylogeographyAndSymbioticEffe.pdf