eprintid: 11667
rev_number: 9
eprint_status: archive
userid: 3170
dir: disk0/00/01/16/67
datestamp: 2020-12-18 06:06:31
lastmod: 2020-12-18 06:06:31
status_changed: 2020-12-18 06:06:31
type: article
metadata_visibility: show
creators_name: Gopalakrishnan, S
creators_name: Sharma, R
creators_name: Srinivas, V
creators_name: Naresh, N
creators_name: Mishra, S P
creators_name: Ankati, S
creators_name: Pratyusha, S
creators_name: Govindaraj, M
creators_name: Gonzalez, S V
creators_name: Nervik, S
creators_name: Simic, N
creators_gender: Female
creators_gender: Female
creators_gender: Female
icrisatcreators_name: Gopalakrishnan, S
icrisatcreators_name: Sharma, R
icrisatcreators_name: Srinivas, V
icrisatcreators_name: Naresh, N
icrisatcreators_name: Mishra, S P
icrisatcreators_name: Ankati, S
icrisatcreators_name: Pratyusha, S
icrisatcreators_name: Govindaraj, M
affiliation: ICRISAT (Patancheru)
affiliation: Department of Chemistry, Faculty of Natural Sciences, Norwegian University of Science and  Technology (NTNU), Trondheim
country: India
country: Norway
title: Identification and Characterization of a Streptomyces albus Strain and Its Secondary Metabolite Organophosphate against Charcoal Rot of Sorghum
ispublished: pub
subjects: s1.4
subjects: s35
divisions: CRPS2
crps: CG1
full_text_status: public
keywords: Macrophomina phaseolina, Charcoal rot, Sorghum, Biocontrol, Organophosphate
note: We thank PVS Prasad for his significant contribution in the laboratory, greenhouse,
and field studies.
abstract: Streptomycesalbus strain CAI-21 has been previously reported to have plant growth-promotion
abilities in chickpea, pigeonpea, rice, and sorghum. The strain CAI-21 and its secondary metabolite
were evaluated for their biocontrol potential against charcoal rot disease in sorghum caused
by Macrophomina phaseolina. Results exhibited that CAI-21 significantly inhibited the growth of
the pathogen, M. phaseolina, in dual-culture (15 mm; zone of inhibition), metabolite production
(74% inhibition), and blotter paper (90% inhibition) assays. When CAI-21 was tested for its biocontrol
potential under greenhouse and field conditions following inoculation of M. phaseolina by toothpick
method, it significantly reduced the number of internodes infected (75% and 45% less, respectively)
and length of infection (75% and 51% less, respectively) over the positive control (only M. phaseolina
inoculated) plants. Under greenhouse conditions, scanning electron microscopic analysis showed
that the phloem and xylem tissues of the CAI-21-treated shoot samples were intact compared to
those of the diseased stem samples. The culture filtrate of the CAI-21 was purified by various
chromatographic techniques, and the active compound was identified as “organophosphate” by
NMR and MS. The e�cacy of organophosphate was found to inhibit the growth of M. phaseolina
in the poisoned food technique. This study indicates that S. albus CAI-21 and its active metabolite
organophosphate have the potential to control charcoal rot in sorghum.
date: 2020-12
date_type: published
publication: Plants (TSI)
volume: 9
number: 12
publisher: MDPI
pagerange: 1-14
id_number: doi:10.3390/plants9121727
refereed: TRUE
issn: 2223-7747
official_url: https://doi.org/10.3390/plants9121727
related_url_url: https://scholar.google.com/scholar?hl=en&as_sdt=0%2C5&q=10.3390%2Fplants9121727&btnG=
related_url_type: pub
funders: CGIAR Research Program on Grain Legumes Dry Land Cereals
citation:   Gopalakrishnan, S and Sharma, R and Srinivas, V and Naresh, N and Mishra, S P and Ankati, S and Pratyusha, S and Govindaraj, M and Gonzalez, S V and Nervik, S and Simic, N  (2020) Identification and Characterization of a Streptomyces albus Strain and Its Secondary Metabolite Organophosphate against Charcoal Rot of Sorghum.  Plants (TSI), 9 (12).  pp. 1-14.  ISSN 2223-7747     
document_url: http://oar.icrisat.org/11667/1/Identification%20and%20Characterization%20CAI-21%20metabolite-SGK%20et%20al.%202020.pdf