%0 Journal Article
%@ 2223-7747
%A Gopalakrishnan, S
%A Sharma, R
%A Srinivas, V
%A Naresh, N
%A Mishra, S P
%A Ankati, S
%A Pratyusha, S
%A Govindaraj, M
%A Gonzalez, S V
%A Nervik, S
%A Simic, N
%D 2020
%F icrisat:11667
%I MDPI
%J Plants (TSI)
%K Macrophomina phaseolina, Charcoal rot, Sorghum, Biocontrol, Organophosphate
%N 12
%P 1-14
%T Identification and Characterization of a Streptomyces albus Strain and Its Secondary Metabolite Organophosphate against Charcoal Rot of Sorghum
%U http://oar.icrisat.org/11667/
%V 9
%X Streptomycesalbus strain CAI-21 has been previously reported to have plant growth-promotion  abilities in chickpea, pigeonpea, rice, and sorghum. The strain CAI-21 and its secondary metabolite  were evaluated for their biocontrol potential against charcoal rot disease in sorghum caused  by Macrophomina phaseolina. Results exhibited that CAI-21 significantly inhibited the growth of  the pathogen, M. phaseolina, in dual-culture (15 mm; zone of inhibition), metabolite production  (74% inhibition), and blotter paper (90% inhibition) assays. When CAI-21 was tested for its biocontrol  potential under greenhouse and field conditions following inoculation of M. phaseolina by toothpick  method, it significantly reduced the number of internodes infected (75% and 45% less, respectively)  and length of infection (75% and 51% less, respectively) over the positive control (only M. phaseolina  inoculated) plants. Under greenhouse conditions, scanning electron microscopic analysis showed  that the phloem and xylem tissues of the CAI-21-treated shoot samples were intact compared to  those of the diseased stem samples. The culture filtrate of the CAI-21 was purified by various  chromatographic techniques, and the active compound was identified as “organophosphate” by  NMR and MS. The e�cacy of organophosphate was found to inhibit the growth of M. phaseolina  in the poisoned food technique. This study indicates that S. albus CAI-21 and its active metabolite  organophosphate have the potential to control charcoal rot in sorghum.
%Z We thank PVS Prasad for his significant contribution in the laboratory, greenhouse,  and field studies.