relation: http://oar.icrisat.org/11667/
title: Identification and Characterization of a Streptomyces albus Strain and Its Secondary Metabolite Organophosphate against Charcoal Rot of Sorghum
creator: Gopalakrishnan, S
creator: Sharma, R
creator: Srinivas, V
creator: Naresh, N
creator: Mishra, S P
creator: Ankati, S
creator: Pratyusha, S
creator: Govindaraj, M
creator: Gonzalez, S V
creator: Nervik, S
creator: Simic, N
subject: Sorghum
subject: Plant Disease
description: Streptomycesalbus strain CAI-21 has been previously reported to have plant growth-promotion  abilities in chickpea, pigeonpea, rice, and sorghum. The strain CAI-21 and its secondary metabolite  were evaluated for their biocontrol potential against charcoal rot disease in sorghum caused  by Macrophomina phaseolina. Results exhibited that CAI-21 significantly inhibited the growth of  the pathogen, M. phaseolina, in dual-culture (15 mm; zone of inhibition), metabolite production  (74% inhibition), and blotter paper (90% inhibition) assays. When CAI-21 was tested for its biocontrol  potential under greenhouse and field conditions following inoculation of M. phaseolina by toothpick  method, it significantly reduced the number of internodes infected (75% and 45% less, respectively)  and length of infection (75% and 51% less, respectively) over the positive control (only M. phaseolina  inoculated) plants. Under greenhouse conditions, scanning electron microscopic analysis showed  that the phloem and xylem tissues of the CAI-21-treated shoot samples were intact compared to  those of the diseased stem samples. The culture filtrate of the CAI-21 was purified by various  chromatographic techniques, and the active compound was identified as “organophosphate” by  NMR and MS. The e�cacy of organophosphate was found to inhibit the growth of M. phaseolina  in the poisoned food technique. This study indicates that S. albus CAI-21 and its active metabolite  organophosphate have the potential to control charcoal rot in sorghum.
publisher: MDPI
date: 2020-12
type: Article
type: PeerReviewed
format: application/pdf
language: en
identifier: http://oar.icrisat.org/11667/1/Identification%20and%20Characterization%20CAI-21%20metabolite-SGK%20et%20al.%202020.pdf
identifier:   Gopalakrishnan, S and Sharma, R and Srinivas, V and Naresh, N and Mishra, S P and Ankati, S and Pratyusha, S and Govindaraj, M and Gonzalez, S V and Nervik, S and Simic, N  (2020) Identification and Characterization of a Streptomyces albus Strain and Its Secondary Metabolite Organophosphate against Charcoal Rot of Sorghum.  Plants (TSI), 9 (12).  pp. 1-14.  ISSN 2223-7747     
relation: https://doi.org/10.3390/plants9121727
relation: doi:10.3390/plants9121727