"11667","9","archive","3170",,,"disk0/00/01/16/67","2020-12-18 06:06:31","2020-12-18 06:06:31","2020-12-18 06:06:31","article",,,"show",,,,"","","","","","","","","","",,,,"Gopalakrishnan","S","","","","","",,"Gopalakrishnan","S","","","","",,,,,"","",,,,,"","","ICRISAT (Patancheru)","India","Identification and Characterization of a Streptomyces albus Strain and Its Secondary Metabolite Organophosphate against Charcoal Rot of Sorghum","pub","s1.4","CRPS2","CG1","public",,,"Macrophomina phaseolina, Charcoal rot, Sorghum, Biocontrol, Organophosphate",,"We thank PVS Prasad for his significant contribution in the laboratory, greenhouse,
and field studies.","Streptomycesalbus strain CAI-21 has been previously reported to have plant growth-promotion
abilities in chickpea, pigeonpea, rice, and sorghum. The strain CAI-21 and its secondary metabolite
were evaluated for their biocontrol potential against charcoal rot disease in sorghum caused
by Macrophomina phaseolina. Results exhibited that CAI-21 significantly inhibited the growth of
the pathogen, M. phaseolina, in dual-culture (15 mm; zone of inhibition), metabolite production
(74% inhibition), and blotter paper (90% inhibition) assays. When CAI-21 was tested for its biocontrol
potential under greenhouse and field conditions following inoculation of M. phaseolina by toothpick
method, it significantly reduced the number of internodes infected (75% and 45% less, respectively)
and length of infection (75% and 51% less, respectively) over the positive control (only M. phaseolina
inoculated) plants. Under greenhouse conditions, scanning electron microscopic analysis showed
that the phloem and xylem tissues of the CAI-21-treated shoot samples were intact compared to
those of the diseased stem samples. The culture filtrate of the CAI-21 was purified by various
chromatographic techniques, and the active compound was identified as “organophosphate” by
NMR and MS. The e�cacy of organophosphate was found to inhibit the growth of M. phaseolina
in the poisoned food technique. This study indicates that S. albus CAI-21 and its active metabolite
organophosphate have the potential to control charcoal rot in sorghum.","2020-12","published",,"Plants (TSI)","9","12","MDPI",,"1-14",,,,,,"doi:10.3390/plants9121727",,,,,"TRUE",,"2223-7747",,,,,,"","https://doi.org/10.3390/plants9121727","https://scholar.google.com/scholar?hl=en&as_sdt=0%2C5&q=10.3390%2Fplants9121727&btnG=","pub",,"CGIAR Research Program on Grain Legumes Dry Land Cereals","",,,,,,"",,,,,,,"",,,,,"",,,,,"","",,,,,"","",,,,,
"11667",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,"Sharma","R","","",,,,,"Sharma","R","","",,,,,,,,,,,,,,,"Department of Chemistry, Faculty of Natural Sciences, Norwegian University of Science and  Technology (NTNU), Trondheim","Norway",,,"s35",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
"11667",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,"Srinivas","V","","",,,,,"Srinivas","V","","",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
"11667",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,"Naresh","N","","",,,,,"Naresh","N","","",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
"11667",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,"Mishra","S P","","",,,,,"Mishra","S P","","",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
"11667",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,"Ankati","S","","",,,,"Female","Ankati","S","","",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
"11667",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,"Pratyusha","S","","",,,,"Female","Pratyusha","S","","",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
"11667",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,"Govindaraj","M","","",,,,,"Govindaraj","M","","",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
"11667",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,"Gonzalez","S V","","",,,,"Female",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
"11667",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,"Nervik","S","","",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
"11667",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,"Simic","N","","",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,