@article{icrisat11667,
           month = {December},
           title = {Identification and Characterization of a Streptomyces albus Strain and Its Secondary Metabolite Organophosphate against Charcoal Rot of Sorghum},
       publisher = {MDPI},
            year = {2020},
          author = {S Gopalakrishnan and R Sharma and V Srinivas and N Naresh and S P Mishra and S Ankati and S Pratyusha and M Govindaraj and S V Gonzalez and S Nervik and N Simic},
           pages = {1--14},
          volume = {9},
            note = {We thank PVS Prasad for his significant contribution in the laboratory, greenhouse,
and field studies.},
         journal = {Plants (TSI)},
          number = {12},
        keywords = {Macrophomina phaseolina, Charcoal rot, Sorghum, Biocontrol, Organophosphate},
             url = {http://oar.icrisat.org/11667/},
        abstract = {Streptomycesalbus strain CAI-21 has been previously reported to have plant growth-promotion
abilities in chickpea, pigeonpea, rice, and sorghum. The strain CAI-21 and its secondary metabolite
were evaluated for their biocontrol potential against charcoal rot disease in sorghum caused
by Macrophomina phaseolina. Results exhibited that CAI-21 significantly inhibited the growth of
the pathogen, M. phaseolina, in dual-culture (15 mm; zone of inhibition), metabolite production
(74\% inhibition), and blotter paper (90\% inhibition) assays. When CAI-21 was tested for its biocontrol
potential under greenhouse and field conditions following inoculation of M. phaseolina by toothpick
method, it significantly reduced the number of internodes infected (75\% and 45\% less, respectively)
and length of infection (75\% and 51\% less, respectively) over the positive control (only M. phaseolina
inoculated) plants. Under greenhouse conditions, scanning electron microscopic analysis showed
that the phloem and xylem tissues of the CAI-21-treated shoot samples were intact compared to
those of the diseased stem samples. The culture filtrate of the CAI-21 was purified by various
chromatographic techniques, and the active compound was identified as ?organophosphate? by
NMR and MS. The e?cacy of organophosphate was found to inhibit the growth of M. phaseolina
in the poisoned food technique. This study indicates that S. albus CAI-21 and its active metabolite
organophosphate have the potential to control charcoal rot in sorghum.}
}