<mets:mets OBJID="eprint_11647" LABEL="Eprints Item" xsi:schemaLocation="http://www.loc.gov/METS/ http://www.loc.gov/standards/mets/mets.xsd http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-3.xsd" xmlns:mets="http://www.loc.gov/METS/" xmlns:mods="http://www.loc.gov/mods/v3" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance"><mets:metsHdr CREATEDATE="2023-07-05T14:09:16Z"><mets:agent ROLE="CUSTODIAN" TYPE="ORGANIZATION"><mets:name>OAR@ICRISAT</mets:name></mets:agent></mets:metsHdr><mets:dmdSec ID="DMD_eprint_11647_mods"><mets:mdWrap MDTYPE="MODS"><mets:xmlData><mods:titleInfo><mods:title>Discovery and validation of candidate genes for grain iron and zinc metabolism in pearl millet [Pennisetum glaucum (L.) R. Br.]</mods:title></mods:titleInfo><mods:name type="personal"><mods:namePart type="given">M D</mods:namePart><mods:namePart type="family">Mahendrakar</mods:namePart><mods:role><mods:roleTerm type="text">author</mods:roleTerm></mods:role></mods:name><mods:name type="personal"><mods:namePart type="given">M</mods:namePart><mods:namePart type="family">Parveda</mods:namePart><mods:role><mods:roleTerm type="text">author</mods:roleTerm></mods:role></mods:name><mods:name type="personal"><mods:namePart type="given">P B K</mods:namePart><mods:namePart type="family">Kishor</mods:namePart><mods:role><mods:roleTerm type="text">author</mods:roleTerm></mods:role></mods:name><mods:name type="personal"><mods:namePart type="given">R K</mods:namePart><mods:namePart type="family">Srivastava</mods:namePart><mods:role><mods:roleTerm type="text">author</mods:roleTerm></mods:role></mods:name><mods:abstract>Pearl millet is an important crop for alleviating micronutrient malnutrition through genomics-assisted&#13;
breeding for grain Fe (GFeC) and Zn (GZnC) content. In this study, we identified candidate genes&#13;
related to iron (Fe) and zinc (Zn) metabolism through gene expression analysis and correlated it with&#13;
known QTL regions for GFeC/GZnC. From a total of 114 Fe and Zn metabolism-related genes that were&#13;
selected from the related crop species, we studied 29 genes. Different developmental stages exhibited&#13;
tissue and stage-specific expressions for Fe and Zn metabolism genes in parents contrasting for GFeC&#13;
and GZnC. Results revealed that PglZIP, PglNRAMP and PglFER gene families were candidates for&#13;
GFeC and GZnC. Ferritin-like gene, PglFER1 may be the potential candidate gene for GFeC. Promoter&#13;
analysis revealed Fe and Zn deficiency, hormone, metal-responsive, and salt-regulated elements.&#13;
Genomic regions underlying GFeC and GZnC were validated by annotating major QTL regions for grain&#13;
Fe and Zn. Interestingly, PglZIP and PglNRAMP gene families were found common with a previously&#13;
reported linkage group 7 major QTL region for GFeC and GZnC. The study provides insights into the&#13;
foundation for functional dissection of different Fe and Zn metabolism genes homologs and their&#13;
subsequent use in pearl millet molecular breeding programs globally.</mods:abstract><mods:classification authority="lcc">Pearl Millet</mods:classification><mods:classification authority="lcc">Genetics and Genomics</mods:classification><mods:classification authority="lcc">Food and Nutrition</mods:classification><mods:originInfo><mods:dateIssued encoding="iso8061">2020-10</mods:dateIssued></mods:originInfo><mods:originInfo><mods:publisher>Nature Research</mods:publisher></mods:originInfo><mods:genre>Article</mods:genre></mets:xmlData></mets:mdWrap></mets:dmdSec><mets:amdSec ID="TMD_eprint_11647"><mets:rightsMD ID="rights_eprint_11647_mods"><mets:mdWrap MDTYPE="MODS"><mets:xmlData><mods:useAndReproduction>
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