"11647","9","archive","3170",,,"disk0/00/01/16/47","2020-11-13 15:13:50","2020-11-13 15:13:50","2020-11-13 15:13:50","article",,,"show",,,,"","","","","","","","","","",,,,"Mahendrakar","M D","","","","","","","Srivastava","R K","","","","",,,,,"","",,,,,"","","ICRISAT (Patancheru)","India","Discovery and validation of candidate genes for grain iron and zinc metabolism in pearl millet [Pennisetum glaucum (L.) R. Br.]","pub","S1.5.1","CRPS3","CG1","public",,,"Pearl Millet, Nutrition",,"The research work was supported by research grants from the CGIAR Research Program on Grain Legumes
and Dryland Cereals (CRP-GLDC), and the Department of Biotechnology (DBT), India. This work has been
published as part of the CGIAR Research Program on Dryland Cereals. Gene expression data and computational
analysis were carried out at the Department of Genetics, Osmania University. PBK is thankful to the CSIR, New
Delhi, for providing CSIR-Emeritus Fellowship and contingency grants [CSIR Reference No. 21(934/12-EMRII)].
This work was also supported by Prof. P.B. Kavi Kishor, who used his personal money to carry out gene
expression experiments. The corresponding authors would like to thank Dr. Nagaraju Marka, Department of
Genetics, who helped in data analysis, Dr. Rajeev Gupta, Dr. Abhishek Rathore, Prasad Bajaj from ICRISAT and
our partners Drs. Rattan S. Yadav and Tara Satyayavathi for their help in research work. We also acknowledge
Dr. Prashanth Suravajhala, and Dr. Purva Lohan, BISR, Jaipur for their inputs. Authors dedicate this manuscript
to Late Vengaldas Rajaram who did all the initial groundwork for this study.","Pearl millet is an important crop for alleviating micronutrient malnutrition through genomics-assisted
breeding for grain Fe (GFeC) and Zn (GZnC) content. In this study, we identified candidate genes
related to iron (Fe) and zinc (Zn) metabolism through gene expression analysis and correlated it with
known QTL regions for GFeC/GZnC. From a total of 114 Fe and Zn metabolism-related genes that were
selected from the related crop species, we studied 29 genes. Different developmental stages exhibited
tissue and stage-specific expressions for Fe and Zn metabolism genes in parents contrasting for GFeC
and GZnC. Results revealed that PglZIP, PglNRAMP and PglFER gene families were candidates for
GFeC and GZnC. Ferritin-like gene, PglFER1 may be the potential candidate gene for GFeC. Promoter
analysis revealed Fe and Zn deficiency, hormone, metal-responsive, and salt-regulated elements.
Genomic regions underlying GFeC and GZnC were validated by annotating major QTL regions for grain
Fe and Zn. Interestingly, PglZIP and PglNRAMP gene families were found common with a previously
reported linkage group 7 major QTL region for GFeC and GZnC. The study provides insights into the
foundation for functional dissection of different Fe and Zn metabolism genes homologs and their
subsequent use in pearl millet molecular breeding programs globally.","2020-10","published",,"Scientific Reports (TSI)","10","1","Nature Research",,,,,,,,"doi:10.1038/s41598-020-73241-7",,,,,"TRUE",,"2045-2322",,,,,,"","https://doi.org/10.1038/s41598-020-73241-7","https://scholar.google.com/scholar?hl=en&as_sdt=0%2C5&q=10.1038%2Fs41598-020-73241-7&btnG=","pub",,"","",,,,,,"",,,,,,,"",,,,,"",,,,,"","",,,,,"","",,,,,
"11647",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,"Parveda","M","","",,,,,,,,,,,,,,,,,,,,,,,"Department of Genetics, Osmania University (OU), Hyderabad, India.",,,,"s2.13",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
"11647",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,"Kishor","P B K","","",,,,,,,,,,,,,,,,,,,,,,,"Department of Biotechnology, Vignan’s Foundation for Science, Technology and Research, Vadlamudi, Guntur,India",,,,"s2.17",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
"11647",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,"Srivastava","R K","","",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,