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        <dc:title>Fine-Mapping of Sorghum Stay-Green QTL on Chromosome10 Revealed Genes Associated with Delayed Senescence</dc:title>
        <dc:creator>Kiranmayee, K N S U</dc:creator>
        <dc:creator>Hash, C T</dc:creator>
        <dc:creator>Sivasubramani, S</dc:creator>
        <dc:creator>Ramu, P</dc:creator>
        <dc:creator>Amindala, B P</dc:creator>
        <dc:creator>Rathore, A</dc:creator>
        <dc:creator>Kishor, P B K</dc:creator>
        <dc:creator>Gupta, R</dc:creator>
        <dc:creator>Deshpande, S P</dc:creator>
        <dc:subject>Sorghum</dc:subject>
        <dc:subject>Genetics and Genomics</dc:subject>
        <dc:description>This study was conducted to dissect the genetic basis and to explore the candidate genes&#13;
underlying one of the important genomic regions on an SBI-10 long arm (L), governing the complex&#13;
stay-green trait contributing to post-flowering drought-tolerance in sorghum. A fine-mapping&#13;
population was developed from an introgression line cross—RSG04008-6 (stay-green)  J2614-11&#13;
(moderately senescent). The fine-mapping population with 1894 F2 was genotyped with eight SSRs&#13;
and a set of 152 recombinants was identified, advanced to the F4 generation, field evaluated with three&#13;
replications over 2 seasons, and genotyped with the GBS approach. A high-resolution linkage map&#13;
was developed for SBI-10L using 260 genotyping by sequencing—Single Nucleotide Polymorphism&#13;
(GBS–SNPs). Using the best linear unpredicted means (BLUPs) of the percent green leaf area (%GL)&#13;
traits and the GBS-based SNPs, weidentified seven quantitative trait loci (QTL) clusters and single gene,&#13;
mostly involved in drought-tolerance, for each QTL cluster, viz., AP2/ERF transcription factor family&#13;
(Sobic.010G202700), NBS-LRR protein (Sobic.010G205600), ankyrin-repeat protein (Sobic.010G205800),&#13;
senescence-associated protein (Sobic.010G270300), WD40 (Sobic.010G205900), CPK1 adapter protein&#13;
(Sobic.010G264400), LEA2 protein (Sobic.010G259200) and an expressed protein (Sobic.010G201100).&#13;
The target genomic region was thus delimited from 15 Mb to 8 genes co-localized with QTL clusters,&#13;
and validated using quantitative real-time (qRT)–PCR.</dc:description>
        <dc:publisher>MDPI</dc:publisher>
        <dc:date>2020-09</dc:date>
        <dc:type>Article</dc:type>
        <dc:type>PeerReviewed</dc:type>
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        <dc:language>en</dc:language>
        <dc:identifier>http://oar.icrisat.org/11643/1/genes-11-01026-v2.pdf</dc:identifier>
        <dc:identifier>  Kiranmayee, K N S U and Hash, C T and Sivasubramani, S and Ramu, P and Amindala, B P and Rathore, A and Kishor, P B K and Gupta, R and Deshpande, S P  (2020) Fine-Mapping of Sorghum Stay-Green QTL on Chromosome10 Revealed Genes Associated with Delayed Senescence.  Genes (TSI), 11 (1026).  pp. 1-26.  ISSN 2073-4425     </dc:identifier>
        <dc:relation>https://doi.org/10.3390/genes11091026</dc:relation>
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