relation: http://oar.icrisat.org/11643/
title: Fine-Mapping of Sorghum Stay-Green QTL on Chromosome10 Revealed Genes Associated with Delayed Senescence
creator: Kiranmayee, K N S U
creator: Hash, C T
creator: Sivasubramani, S
creator: Ramu, P
creator: Amindala, B P
creator: Rathore, A
creator: Kishor, P B K
creator: Gupta, R
creator: Deshpande, S P
subject: Sorghum
subject: Genetics and Genomics
description: This study was conducted to dissect the genetic basis and to explore the candidate genes  underlying one of the important genomic regions on an SBI-10 long arm (L), governing the complex  stay-green trait contributing to post-flowering drought-tolerance in sorghum. A fine-mapping  population was developed from an introgression line cross—RSG04008-6 (stay-green)  J2614-11  (moderately senescent). The fine-mapping population with 1894 F2 was genotyped with eight SSRs  and a set of 152 recombinants was identified, advanced to the F4 generation, field evaluated with three  replications over 2 seasons, and genotyped with the GBS approach. A high-resolution linkage map  was developed for SBI-10L using 260 genotyping by sequencing—Single Nucleotide Polymorphism  (GBS–SNPs). Using the best linear unpredicted means (BLUPs) of the percent green leaf area (%GL)  traits and the GBS-based SNPs, weidentified seven quantitative trait loci (QTL) clusters and single gene,  mostly involved in drought-tolerance, for each QTL cluster, viz., AP2/ERF transcription factor family  (Sobic.010G202700), NBS-LRR protein (Sobic.010G205600), ankyrin-repeat protein (Sobic.010G205800),  senescence-associated protein (Sobic.010G270300), WD40 (Sobic.010G205900), CPK1 adapter protein  (Sobic.010G264400), LEA2 protein (Sobic.010G259200) and an expressed protein (Sobic.010G201100).  The target genomic region was thus delimited from 15 Mb to 8 genes co-localized with QTL clusters,  and validated using quantitative real-time (qRT)–PCR.
publisher: MDPI
date: 2020-09
type: Article
type: PeerReviewed
format: application/pdf
language: en
identifier: http://oar.icrisat.org/11643/1/genes-11-01026-v2.pdf
identifier:   Kiranmayee, K N S U and Hash, C T and Sivasubramani, S and Ramu, P and Amindala, B P and Rathore, A and Kishor, P B K and Gupta, R and Deshpande, S P  (2020) Fine-Mapping of Sorghum Stay-Green QTL on Chromosome10 Revealed Genes Associated with Delayed Senescence.  Genes (TSI), 11 (1026).  pp. 1-26.  ISSN 2073-4425     
relation: https://doi.org/10.3390/genes11091026
relation: doi:10.3390/genes11091026