"11643","13","archive","3170",,,"disk0/00/01/16/43","2020-11-13 05:42:19","2021-08-20 08:44:24","2020-11-13 05:42:19","article",,,"show",,,,"","","","","","","","","","",,,,"Kiranmayee","K N S U","","","","","","Female","Kiranmayee","K N S U","","","","",,,,,"","",,,,,"","","ICRISAT (Patancheru)","India","Fine-Mapping of Sorghum Stay-Green QTL on Chromosome10 Revealed Genes Associated with Delayed Senescence","pub","s1.4","CRPS3","CG1","public",,,"sorghum chromosome 10 long arm (SBI-10L), fine-mapping, Stay-green, Candidate genes, Percent green leaf area, Delayed senescence",,"This work is a part of the Ph.D. and post-doctoral research work of the first author. The authors
are thankful to C. Muralidhar for his help in conducting field experiments. Funding support to R.G. and S.P.D. from
the Department of Biotechnology (grant# BT-IN-UK-VNC-42-RG-2015-16), Government of India, the Biotechnology
and Biological Sciences Research Council (GCRF/BBSRC grant # BB/P027970/1), United Kingdom, and the CGIAR
Research Program on Grain Legumes and Dryland Cereals (CRP-GLDC) is gratefully acknowledged. ICRISAT is
a member of the CGIAR consortium. PBK is grateful to the VFSTR, Guntur, for providing fellowship.","This study was conducted to dissect the genetic basis and to explore the candidate genes
underlying one of the important genomic regions on an SBI-10 long arm (L), governing the complex
stay-green trait contributing to post-flowering drought-tolerance in sorghum. A fine-mapping
population was developed from an introgression line cross—RSG04008-6 (stay-green)  J2614-11
(moderately senescent). The fine-mapping population with 1894 F2 was genotyped with eight SSRs
and a set of 152 recombinants was identified, advanced to the F4 generation, field evaluated with three
replications over 2 seasons, and genotyped with the GBS approach. A high-resolution linkage map
was developed for SBI-10L using 260 genotyping by sequencing—Single Nucleotide Polymorphism
(GBS–SNPs). Using the best linear unpredicted means (BLUPs) of the percent green leaf area (%GL)
traits and the GBS-based SNPs, weidentified seven quantitative trait loci (QTL) clusters and single gene,
mostly involved in drought-tolerance, for each QTL cluster, viz., AP2/ERF transcription factor family
(Sobic.010G202700), NBS-LRR protein (Sobic.010G205600), ankyrin-repeat protein (Sobic.010G205800),
senescence-associated protein (Sobic.010G270300), WD40 (Sobic.010G205900), CPK1 adapter protein
(Sobic.010G264400), LEA2 protein (Sobic.010G259200) and an expressed protein (Sobic.010G201100).
The target genomic region was thus delimited from 15 Mb to 8 genes co-localized with QTL clusters,
and validated using quantitative real-time (qRT)–PCR.","2020-09","published",,"Genes (TSI)","11","1026","MDPI",,"1-26",,,,,,"doi:10.3390/genes11091026",,,,,"TRUE",,"2073-4425",,,,,,"","https://doi.org/10.3390/genes11091026","https://scholar.google.com/scholar?hl=en&as_sdt=0%2C5&q=10.3390%2Fgenes11091026&btnG=","pub",,"","",,,,,,"",,,,,,,"",,,,,"",,,,,"","",,,,,"","",,,,,
"11643",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,"Hash","C T","","",,,,,"Sivasubramani","S","","",,,,,,,,,,,,,,,"Department of Genetics, Faculty of Sciences, Osmania University, Hyderabad, Telangana","Niger",,,"s2.13","CRPS1",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
"11643",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,"Sivasubramani","S","","",,,,,"Ramu","P","","",,,,,,,,,,,,,,,"ICRISAT (Niamey)",,,,,"S15",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
"11643",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,"Ramu","P","","",,,,,"Amindala","B P","","",,,,,,,,,,,,,,,"Department of Biotechnology, Vignan’s Foundation for Science, Technology & Research, Vadlamudi, Guntur, Andhra Pradesh",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
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