relation: http://oar.icrisat.org/11638/
title: Genome-wide association study uncovers genomic regions associated with grain iron, zinc and protein content in pearl millet
creator: Pujar, M
creator: Gangaprasad, S
creator: Govindaraj, M
creator: Gangurde, S S
creator: Kanatti, A
creator: Kudapa, H
subject: Pearl Millet
subject: Food and Nutrition
description: Pearl millet hybrids biofortified with iron (Fe) and zinc (Zn) promise to be part of a long-term strategy  to combat micronutrient malnutrition in the arid and semi-arid tropical (SAT) regions of the world.  Biofortification through molecular breeding is the way forward to achieving a rapid trait-based  breeding strategy. This genome-wide association study (GWAS) was conducted to identify significant  marker-trait associations (MTAs) for Fe, Zn, and protein content (PC) for enhanced biofortification  breeding. A diverse panel of 281 advanced inbred lines was evaluated for Fe, Zn, and PC over two  seasons. Phenotypic evaluation revealed high variability (Fe: 32–120 mg kg−1, Zn: 19–87 mg kg−1, PC:  8–16%), heritability (hbs2 ≥ 90%) and significantly positive correlation among Fe, Zn and PC (P = 0.01),  implying concurrent improvement. Based on the Diversity Arrays Technology (DArT) seq assay, 58,719  highly informative SNPs were filtered for association mapping. Population structure analysis showed  six major genetic groups (K = 6). A total of 78 MTAs were identified, of which 18 were associated with  Fe, 43 with Zn, and 17 with PC. Four SNPs viz., Pgl04_64673688, Pgl05_135500493, Pgl05_144482656,  and Pgl07_101483782 located on chromosomes Pgl04 (1), Pgl05 (2) and Pgl07 (1), respectively were  co-segregated for Fe and Zn. Promising genes, ‘Late embryogenesis abundant protein’, ‘Myb domain’,  ‘pentatricopeptide repeat’, and ‘iron ion binding’ coded by 8 SNPs were identified. The SNPs/genes  identified in the present study presents prospects for genomics assisted biofortification breeding in  pearl millet.
publisher: Nature Research
date: 2020-11
type: Article
type: PeerReviewed
format: application/pdf
language: en
identifier: http://oar.icrisat.org/11638/1/GWAS%20Scientific%20reports%20s41598-020-76230-y.pdf
identifier:   Pujar, M and Gangaprasad, S and Govindaraj, M and Gangurde, S S and Kanatti, A and Kudapa, H  (2020) Genome-wide association study uncovers genomic regions associated with grain iron, zinc and protein content in pearl millet.  Scientific Reports (TSI), 10 (1).   ISSN 2045-2322     
relation: https://doi.org/10.1038/s41598-020-76230-y
relation: doi:10.1038/s41598-020-76230-y