"11638","7","archive","3170",,,"disk0/00/01/16/38","2020-11-10 15:00:56","2020-11-10 15:00:56","2020-11-10 15:00:56","article",,,"show",,,,"","","","","","","","","","",,,,"Pujar","M","","","","","",,"Govindaraj","M","","","","",,,,,"","",,,,,"","","ICRISAT (Patancheru)","India","Genome-wide association study uncovers genomic regions associated with grain iron, zinc and protein content in pearl millet","pub","S1.5.1","CRPS2","crp1.15","public",,,"Pearl millet, Nutrition",,"This research was supported by funding from the HarvestPlus Challenge Program of the CGIAR. It was carried
out as part of the CGIAR Research Program (CRP) on Agriculture for Nutrition and Health.","Pearl millet hybrids biofortified with iron (Fe) and zinc (Zn) promise to be part of a long-term strategy
to combat micronutrient malnutrition in the arid and semi-arid tropical (SAT) regions of the world.
Biofortification through molecular breeding is the way forward to achieving a rapid trait-based
breeding strategy. This genome-wide association study (GWAS) was conducted to identify significant
marker-trait associations (MTAs) for Fe, Zn, and protein content (PC) for enhanced biofortification
breeding. A diverse panel of 281 advanced inbred lines was evaluated for Fe, Zn, and PC over two
seasons. Phenotypic evaluation revealed high variability (Fe: 32–120 mg kg−1, Zn: 19–87 mg kg−1, PC:
8–16%), heritability (hbs2 ≥ 90%) and significantly positive correlation among Fe, Zn and PC (P = 0.01),
implying concurrent improvement. Based on the Diversity Arrays Technology (DArT) seq assay, 58,719
highly informative SNPs were filtered for association mapping. Population structure analysis showed
six major genetic groups (K = 6). A total of 78 MTAs were identified, of which 18 were associated with
Fe, 43 with Zn, and 17 with PC. Four SNPs viz., Pgl04_64673688, Pgl05_135500493, Pgl05_144482656,
and Pgl07_101483782 located on chromosomes Pgl04 (1), Pgl05 (2) and Pgl07 (1), respectively were
co-segregated for Fe and Zn. Promising genes, ‘Late embryogenesis abundant protein’, ‘Myb domain’,
‘pentatricopeptide repeat’, and ‘iron ion binding’ coded by 8 SNPs were identified. The SNPs/genes
identified in the present study presents prospects for genomics assisted biofortification breeding in
pearl millet.","2020-11","published",,"Scientific Reports (TSI)","10","1","Nature Research",,,,,,,,"doi:10.1038/s41598-020-76230-y",,,,,"TRUE",,"2045-2322",,,,,,"","https://doi.org/10.1038/s41598-020-76230-y","","",,"","",,,,,,"",,,,,,,"",,,,,"",,,,,"","",,,,,"","",,,,,
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