eprintid: 11620 rev_number: 12 eprint_status: archive userid: 3170 dir: disk0/00/01/16/20 datestamp: 2020-09-13 12:26:38 lastmod: 2020-09-17 02:05:35 status_changed: 2020-09-13 12:26:38 type: article metadata_visibility: show creators_name: Sivasakthi, K creators_name: Tharanya, M creators_name: Zaman‐Allah, M creators_name: Kholová, J creators_name: Thirunalasundari, T creators_name: Vadez, V creators_gender: Female creators_gender: Female creators_gender: Female creators_gender: Female icrisatcreators_name: Sivasakthi, K icrisatcreators_name: Tharanya, M icrisatcreators_name: Kholová, J icrisatcreators_name: Vadez, V affiliation: ICRISAT (Patancheru) affiliation: Department of Industrial Biotechnology, Bharathidasan University, Tiruchirappalli, Tamilnadu affiliation: International Center for Maize and Wheat Improvement (CIMMYT), Mount Pleasant Harare affiliation: IRD (Institut de Recherche pour le Developpement) – Univ. Montpellier – UMR DIADE, Montpellier country: India country: Zimbabwe country: France title: Transpiration difference under high evaporative demand in chickpea ( Cicer arietinum L.) may be explained by differences in the water transport pathway in the root cylinder ispublished: pub subjects: S2 subjects: s1.1 subjects: s1000 subjects: wa1 divisions: CRPS4 crps: CG1 full_text_status: public keywords: Apoplastic pathway, Aquaporins, Cell-to-cell pathway, Drought, Hydraulics, Roots, Water saving note: The authors acknowledge support from ICRISAT for a Blue Sky Research Project on ‘Developing crops with high productivity at high temperatures’, and the CGIAR Research Program (CRP) on Grain Legumes. The writing of this paper was supported by the Make Our Planet Great Again (MOPGA) ICARUS project (Improve Crops in Arid Regions and Future Climates) funded by the Agence Nationale de la Recherche (ANR, grant ANR-17-MPGA-0011). Special thanks to Mrs. Rekha Baddam and Mr. Mathew V. Joseph for help with statistical analysis and growth chamber operations. abstract: Terminal drought substantially reduces chickpea yield. Reducing water use at vegetative stage by reducing transpiration under high vapor pressure deficit (VPD), i.e. under dry/ hot conditions, contributes to drought adaptation. We hypothesized that this trait could relate to differences in a genotype’s dependence on root water transport pathways and hydraulics. • Transpiration rate responses in conservative and profligate chickpea genotypes were evaluated under increasing VPD in the presence/absence of apoplastic and cell-to-cell transport inhibitors. • Conservative genotypes ICC 4958 and ICC 8058 restricted transpiration under high VPD compared to the profligate genotypes ICC 14799 and ICC 867. Profligate genotypes were more affected by aquaporin inhibition of the cell-to-cell pathway than conservative genotypes, as measured by the root hydraulic conductance and transpiration under high VPD. Aquaporin inhibitor treatment also led to a larger reduction in root hydraulic conductivity in profligate than in conservative genotypes. In contrast, blockage of the apoplastic pathway in roots decreased transpiration more in conservative than in profligate genotypes. Interestingly, conservative genotypes had high early vigour, whereas profligate genotypes had low early vigour. • In conclusion, profligate genotypes depend more on the cell-to-cell pathway, which might explain their higher root hydraulic conductivity, whereas water-saving by restricting transpiration led to higher dependence on the apoplastic pathway. This opens the possibility to screen for conservative or profligate chickpea phenotypes using inhibitors, itself opening to the search of the genetic basis of these differences. date: 2020-06 date_type: published publication: Plant Biology (TSI) publisher: Wiley pagerange: 769-780 id_number: doi:10.1111/plb.13147 refereed: TRUE issn: 1435-8603 official_url: https://doi.org/10.1111/plb.13147 related_url_url: https://scholar.google.com/scholar?hl=en&as_sdt=0%2C5&q=10.1111%2Fplb.13147&btnG= related_url_type: pub citation: Sivasakthi, K and Tharanya, M and Zaman‐Allah, M and Kholová, J and Thirunalasundari, T and Vadez, V (2020) Transpiration difference under high evaporative demand in chickpea ( Cicer arietinum L.) may be explained by differences in the water transport pathway in the root cylinder. Plant Biology (TSI). pp. 769-780. ISSN 1435-8603 document_url: http://oar.icrisat.org/11620/1/plb.13147.pdf