Fatty acid desaturase-2 (ahFAD2) mutant alleles in peanut (Arachis hypogaea L.) pre-breeding lines: an insight into the source, features, discourse, and selection of novel pre-breeding linesJ HKamdarauthorM DJasaniauthorB CAjayauthorKRaniauthorNManivannanauthorR PVasanthiauthorK LDobariyaauthorM KPandeyauthorPJanilaauthorTRadhakrishnanauthorR KVarshneyauthorS KBeraauthorHigh oleic peanuts and derived food
products offer longer shelf life benefits to the food
processing industry in addition to multiple health
benefits to the consumers. The two mutant alleles,
ahFAD2A and ahFAD2B control composition of oleic,
linoleic and palmitic acid content in peanut. A total of
563 peanut pre-breeding lines were tested for the
presence ahFAD2A and ahFAD2B mutant alleles
using allele specific markers. The ahFAD2A mutant
allele was present in 82 lines, while none of these lines
had ahFAD2B mutant allele. Among botanical types,
ahFAD2A mutant allele was more frequent in lines
with Virginia growth habit than Spanish bunch
although no correlation of ahFAD2A mutant allele
with high oleic acid content and growth habit could be
established. Oleic and linoleic acid content in 82 prebreeding
lines ranged from 39.70 to 62.70% and 17.76 to 31.95%, respectively, with maximum oleic to
linoleic acid ratio of 4. Oleic acid was found to be
negatively correlated with linoleic and palmitic acid.
Further, pre-breeding lines with ahFAD2A mutant
allele, high oleic content and high oleic to linoleic
ratio were investigated and novel lines were identified
for resistance to late leaf spot, short duration, higher
pod yield and other yield related traits. These novel
pre-breeding lines can be used as a potential donor in
peanut improvement programme and to diversify the
primary gene pool including initiating further research
on induction of fresh ahFAD2B mutant allele.Plant BreedingGroundnutGenetics and Genomics2020-08SpringerArticle