eprintid: 11617
rev_number: 11
eprint_status: archive
userid: 3170
dir: disk0/00/01/16/17
datestamp: 2020-09-08 06:05:48
lastmod: 2020-09-08 06:05:48
status_changed: 2020-09-08 06:05:48
type: article
metadata_visibility: show
creators_name: Khan, S A
creators_name: Chen, H
creators_name: Deng, Y
creators_name: Chen, Y
creators_name: Zhang, C
creators_name: Cai, T
creators_name: Ali, N
creators_name: Mamadou, G
creators_name: Xie, D
creators_name: Guo, B
creators_name: Varshney, R K
creators_name: Zhuang, W
icrisatcreators_name: Varshney, R K
affiliation: Fujian Provincial Key Laboratory of Plant Molecular and Cell Biology, State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fujian Agriculture and Forestry University, Fuzhou, Fujian, China
affiliation: College of Crop Science, Fujian Agriculture and Forestry University, Fuzhou
affiliation: College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou
affiliation: Crop Protection and Management Research Unit, USDA-ARS, Tifton
affiliation: ICRISAT (Patancheru)
country: China
country: USA
country: India
title: High-density SNP map facilitates fine mapping of QTLs and candidate genes discovery for Aspergillus flavus resistance in peanut (Arachis hypogaea)
ispublished: pub
subjects: s1.3
subjects: s2.13
subjects: s35
subjects: s40051
divisions: CRPS3
full_text_status: public
keywords: Groundnut, Aflatoxin, Genetics, Genomics
note: Current work was supported by The National Science
Foundation of People’s Republic of China (U1705233; 31601337;
31701463). The authors are thankful to Fujian Key Laboratory of Plant
Molecular and Cell Biology, Fujian Agriculture and Forestry University,
Fuzhou, Fujian, 350002, China, for providing research facilities
and technical guidance. Special acknowledgment to Meng Yang from
Nextomics Biosciences Institute, Wuhan, China for his help in bioinformatics
analysis.
abstract: Aflatoxin contamination in peanuts caused by Aspergillus flavus is a serious food safety issue for human health
around the world. Host plant resistance to fungal infection and reduction in aflatoxin are crucial for mitigating this problem.
Identification of the resistance-linked markers can be used in marker-assisted breeding for varietal development. Here we
report construction of two high-density genetic linkage maps with 1975 SNP loci and 5022 SNP loci, respectively. Two
consistent quantitative trait loci (QTL) were identified as qRAF-3-1 and qRAF-14-1, which located on chromosomes A03
and B04, respectively. QTL qRAF-3-1 was mapped within 1.67 cM and had more than 19% phenotypic variance explained
(PVE), while qRAF-14-1 was located within 1.34 cM with 5.15% PVE. While comparing with the reference genome, the
mapped QTLs, qRAF-3-1 and qRAF-14-1, were located within a physical distance of 1.44 Megabase pair (Mbp) and 2.22
Mbp, harboring 67 and 137 genes, respectively. Among the identified candidate genes, six genes with the same function were
found within both QTLs regions. In addition, putative disease resistance RPP13-like protein 1 (RPP13), lipoxygenase (Lox),
WRKY transcription factor (WRKY) and cytochrome P450 71B34 genes were also identified. Using microarray analysis,
genes responded to A. flavus infection included coding for RPP13, pentatricopeptide repeat-containing-like protein, and
Lox which may be possible candidate genes for resistance to A. flavus. The QTLs and candidate genes will further facilitate
marker development and validation of genes for deployment in the molecular breeding programs against A. flavus in peanuts.
date: 2020-04
date_type: published
publication: Theoretical and Applied Genetics (TSI)
volume: 133
number: 7
publisher: SPRINGER
pagerange: 2239-2257
id_number: doi:10.1007/s00122-020-03594-0
refereed: TRUE
issn: 0040-5752
official_url: https://doi.org/10.1007/s00122-020-03594-0
citation:   Khan, S A and Chen, H and Deng, Y and Chen, Y and Zhang, C and Cai, T and Ali, N and Mamadou, G and Xie, D and Guo, B and Varshney, R K and Zhuang, W  (2020) High-density SNP map facilitates fine mapping of QTLs and candidate genes discovery for Aspergillus flavus resistance in peanut (Arachis hypogaea).  Theoretical and Applied Genetics (TSI), 133 (7).  pp. 2239-2257.  ISSN 0040-5752     
document_url: http://oar.icrisat.org/11617/1/s00122-020-03594-0-3.pdf