relation: http://oar.icrisat.org/11613/ title: Genotyping-by-sequencing and multilocation evaluation of two interspecific backcross populations identify QTLs for yield-related traits in pigeonpea creator: Saxena, R K creator: Kale, S creator: Mir, R R creator: Mallikarjuna, N creator: Yadav, P creator: Das, R R creator: Molla, J creator: Sonnappa, M creator: Ghanta, A creator: Narasimhan, Y creator: Rathore, A creator: Kumar, C V S creator: Varshney, R K subject: Pigeonpea subject: Genetics and Genomics description: This study has identified single-nucleotide polymorphism (SNP) markers associated with nine yield-related traits in pigeonpea by using two backcross populations (BP) developed through interspecific crosses and evaluating them at two locations and 3 years. In both the populations, markers have shown strong segregation distortion; therefore, a quantitative trait locus (QTL) mapping mixed model was used. A total of 86 QTLs explaining 12–21% phenotypic variation were detected in BP-1. On the other hand, 107 QTLs explaining 11–29% phenotypic variation were detected in BP-2. Although most QTLs were environment and trait specific, few stable and consistent QTLs were also detected. Interestingly, 11 QTLs in BP-2 were associated with more than one trait. Among these QTLs, eight QTLs associated with days to 50% flowering and days to 75% maturity were located on CcLG07. One SNP “S7_14185076” marker in BP-2 population has been found associated with four traits, namely days to 50% flowering, days to 75% maturity, primary branches per plant and secondary branches per plant with positive additive effect. Hence, the present study has not only identified QTLs for yield-related traits, but also discovered novel alleles from wild species, which can be used for improvement of traits through genomics-assisted breeding. publisher: SPRINGER date: 2019-12 type: Article type: PeerReviewed format: application/pdf language: en identifier: http://oar.icrisat.org/11613/1/s00122-019-03504-z-1.pdf identifier: Saxena, R K and Kale, S and Mir, R R and Mallikarjuna, N and Yadav, P and Das, R R and Molla, J and Sonnappa, M and Ghanta, A and Narasimhan, Y and Rathore, A and Kumar, C V S and Varshney, R K (2019) Genotyping-by-sequencing and multilocation evaluation of two interspecific backcross populations identify QTLs for yield-related traits in pigeonpea. Theoretical and Applied Genetics (TSI), 133 (3). pp. 737-749. ISSN 0040-5752 relation: https://doi.org/10.1007/s00122-019-03504-z relation: doi:10.1007/s00122-019-03504-z