%0 Journal Article
%@ 1369-5266
%A Varshney, R K
%A Sinha, P
%A Singh, V K
%A Kumar, A
%A Zhang, Q
%A Bennetzen, J L
%D 2020
%F icrisat:11597
%I Elsevier
%J Current Opinion in Plant Biology (TSI)
%K Genome assembly, Germplasm characterization, Gene  function identification, Genomic breeding,Gene  editing
%P 190-196
%T 5Gs for crop genetic improvement
%U http://oar.icrisat.org/11597/
%V 56
%X Here we propose a 5G breeding approach for bringing muchneeded  disruptive changes to crop improvement. These 5Gs  are Genome assembly, Germplasm characterization, Gene  function identification, Genomic breeding (GB), and Gene  editing (GE). In our view, it is important to have genome  assemblies available for each crop and a deep collection of  germplasm characterized at sequencing and agronomic levels  for identification of marker-trait associations and superior  haplotypes. Systems biology and sequencing-based mapping  approaches can be used to identify genes involved in pathways  leading to the expression of a trait, thereby providing diagnostic  markers for target traits. These genes, markers, haplotypes,  and genome-wide sequencing data may be utilized in GB and  GE methodologies in combination with a rapid cycle breeding  strategy.
%Z We are grateful to colleagues for exchanging ideas and discussions related to  the contents of this article. Our sincere apologies to the authors whose work  was not mentioned here due to limited space. RKV thanks the Science &  Engineering Research Board (SERB) of the Department of Science &  Technology (DST), Government of India for providing the J C Bose  National Fellowship (SB/S9/Z-13/2019) and also the Bill and Melinda Gates  Foundation and CGIAR Research Program on Grain Legumes and Dryland  Cereals (GLDC) for partial funding support. ICRISAT is a member of the  CGIAR.