eprintid: 11589
rev_number: 7
eprint_status: archive
userid: 3170
dir: disk0/00/01/15/89
datestamp: 2020-09-04 17:03:35
lastmod: 2020-09-04 17:03:35
status_changed: 2020-09-04 17:03:35
type: article
metadata_visibility: show
creators_name: Baisakh, N
creators_name: Sachdeva, S
creators_name: Bharadwaj, C
creators_name: Singh, R K
creators_name: Jain, P K
creators_name: Patil, B S
creators_name: Roorkiwal, M
creators_name: Varshney, R K
creators_gender: Female
icrisatcreators_name: Roorkiwal, M
icrisatcreators_name: Varshney, R K
affiliation: Division of Genetics, Indian Agricultural Research Institute (ICAR), Pusa, New Delhi, India
affiliation: National Institute of Plant Biotechnology (ICAR), Pusa, New Delhi
affiliation: ICRISAT (Patancheru)
country: India
title: Characterization of ASR gene and its role in drought tolerance in chickpea (Cicer arietinum L.)
ispublished: pub
subjects: s1.1
subjects: s2.13
divisions: CRPS3
full_text_status: public
keywords: Chickpea, Genetics, Genomics
note: The authors would like to thank the Division of Genetics for providing all the facilities to carry
out genotyping and software analysis.
abstract: Chickpea has a profound nutritional and economic value in vegetarian society. Continuous
decline in chickpea productivity is attributed to insufficient genetic variability and different
environmental stresses. Chickpea like several other legumes is highly susceptible to terminal
drought stress. Multiple genes control drought tolerance and ASR gene plays a key role
in regulating different plant stresses. The present study describes the molecular characterization
and functional role of Abscissic acid and stress ripening (ASR) gene from chickpea
(Cicer arietinum) and the gene sequence identified was submitted to NCBI Genbank
(MK937569). Molecular analysis using MUSCLE software proved that the ASR nucleotide
sequences in different legumes show variations at various positions though ASR genes are
conserved in chickpea with only few variations. Sequence similarity of ASR gene to chickpea
putative ABA/WDS induced protein mRNA clearly indicated its potential involvement in
drought tolerance. Physiological screening and qRT-PCR results demonstrated increased
ASR gene expression under drought stress possibly enabled genotypes to perform better
under stress. Conserved domain search, protein structure analysis, prediction and validation,
network analysis using Phyre2, Swiss-PDB viewer, ProSA and STRING analysis
established the role of hypothetical ASR protein NP_001351739.1 in mediating drought
responses. NP_001351739.1 might have enhanced the ASR gene activity as a transcription
factor regulating drought stress tolerance in chickpea. This study could be useful in identification
of new ASR genes that play a major role in drought tolerance and also develop functional
markers for chickpea improvement.
date: 2020-07
date_type: published
publication: PLOS ONE (TSI)
volume: 15
number: 7
publisher: Public Library of Science
pagerange: 1-25
id_number: doi:10.1371/journal.pone.0234550
refereed: TRUE
issn: 1932-6203
official_url: https://doi.org/10.1371/journal.pone.0234550
related_url_url: https://scholar.google.com/scholar?hl=en&as_sdt=0%2C5&q=10.1371%2Fjournal.pone.0234550&btnG=
related_url_type: pub
citation:   Baisakh, N and Sachdeva, S and Bharadwaj, C and Singh, R K and Jain, P K and Patil, B S and Roorkiwal, M and Varshney, R K  (2020) Characterization of ASR gene and its role in drought tolerance in chickpea (Cicer arietinum L.).  PLOS ONE (TSI), 15 (7).  pp. 1-25.  ISSN 1932-6203     
document_url: http://oar.icrisat.org/11589/1/file.pdf