"11589","7","archive","3170",,,"disk0/00/01/15/89","2020-09-04 17:03:35","2020-09-04 17:03:35","2020-09-04 17:03:35","article",,,"show",,,,"","","","","","","","","","",,,,"Baisakh","N","","","","","",,"Roorkiwal","M","","","","",,,,,"","",,,,,"","","Division of Genetics, Indian Agricultural Research Institute (ICAR), Pusa, New Delhi, India","India","Characterization of ASR gene and its role in drought tolerance in chickpea (Cicer arietinum L.)","pub","s1.1","CRPS3","","public",,,"Chickpea, Genetics, Genomics",,"The authors would like to thank the Division of Genetics for providing all the facilities to carry
out genotyping and software analysis.","Chickpea has a profound nutritional and economic value in vegetarian society. Continuous
decline in chickpea productivity is attributed to insufficient genetic variability and different
environmental stresses. Chickpea like several other legumes is highly susceptible to terminal
drought stress. Multiple genes control drought tolerance and ASR gene plays a key role
in regulating different plant stresses. The present study describes the molecular characterization
and functional role of Abscissic acid and stress ripening (ASR) gene from chickpea
(Cicer arietinum) and the gene sequence identified was submitted to NCBI Genbank
(MK937569). Molecular analysis using MUSCLE software proved that the ASR nucleotide
sequences in different legumes show variations at various positions though ASR genes are
conserved in chickpea with only few variations. Sequence similarity of ASR gene to chickpea
putative ABA/WDS induced protein mRNA clearly indicated its potential involvement in
drought tolerance. Physiological screening and qRT-PCR results demonstrated increased
ASR gene expression under drought stress possibly enabled genotypes to perform better
under stress. Conserved domain search, protein structure analysis, prediction and validation,
network analysis using Phyre2, Swiss-PDB viewer, ProSA and STRING analysis
established the role of hypothetical ASR protein NP_001351739.1 in mediating drought
responses. NP_001351739.1 might have enhanced the ASR gene activity as a transcription
factor regulating drought stress tolerance in chickpea. This study could be useful in identification
of new ASR genes that play a major role in drought tolerance and also develop functional
markers for chickpea improvement.","2020-07","published",,"PLOS ONE (TSI)","15","7","Public Library of Science",,"1-25",,,,,,"doi:10.1371/journal.pone.0234550",,,,,"TRUE",,"1932-6203",,,,,,"","https://doi.org/10.1371/journal.pone.0234550","https://scholar.google.com/scholar?hl=en&as_sdt=0%2C5&q=10.1371%2Fjournal.pone.0234550&btnG=","pub",,"","",,,,,,"",,,,,,,"",,,,,"",,,,,"","",,,,,"","",,,,,
"11589",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,"Sachdeva","S","","",,,,"Female","Varshney","R K","","",,,,,,,,,,,,,,,"National Institute of Plant Biotechnology (ICAR), Pusa, New Delhi",,,,"s2.13",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
"11589",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,"Bharadwaj","C","","",,,,,,,,,,,,,,,,,,,,,,,"ICRISAT (Patancheru)",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
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"11589",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,"Jain","P K","","",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
"11589",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,"Patil","B S","","",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
"11589",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,"Roorkiwal","M","","",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
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