<mods:mods version="3.3" xsi:schemaLocation="http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-3.xsd" xmlns:mods="http://www.loc.gov/mods/v3" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance"><mods:titleInfo><mods:title>Fine mapping and gene cloning in the post-NGS era: advances and prospects</mods:title></mods:titleInfo><mods:name type="personal"><mods:namePart type="given">D</mods:namePart><mods:namePart type="family">Jaganathan</mods:namePart><mods:role><mods:roleTerm type="text">author</mods:roleTerm></mods:role></mods:name><mods:name type="personal"><mods:namePart type="given">A</mods:namePart><mods:namePart type="family">Bohra</mods:namePart><mods:role><mods:roleTerm type="text">author</mods:roleTerm></mods:role></mods:name><mods:name type="personal"><mods:namePart type="given">M</mods:namePart><mods:namePart type="family">Thudi</mods:namePart><mods:role><mods:roleTerm type="text">author</mods:roleTerm></mods:role></mods:name><mods:name type="personal"><mods:namePart type="given">R K</mods:namePart><mods:namePart type="family">Varshney</mods:namePart><mods:role><mods:roleTerm type="text">author</mods:roleTerm></mods:role></mods:name><mods:abstract>Improvement in traits of agronomic importance is the top breeding priority of crop improvement programs. Majority of&#13;
these agronomic traits show complex quantitative inheritance. Identification of quantitative trait loci (QTLs) followed by&#13;
fine mapping QTLs and cloning of candidate genes/QTLs is central to trait analysis. Advances in genomic technologies&#13;
revolutionized our understanding of genetics of complex traits, and genomic regions associated with traits were employed in&#13;
marker-assisted breeding or cloning of QTLs/genes. Next-generation sequencing (NGS) technologies have enabled genomewide&#13;
methodologies for the development of ultra-high-density genetic linkage maps in different crops, thus allowing placement&#13;
of candidate loci within few kbs in genomes. In this review, we compare the marker systems used for fine mapping and&#13;
QTL cloning in the pre- and post-NGS era. We then discuss how different NGS platforms in combination with advanced&#13;
experimental designs have improved trait analysis and fine mapping. We opine that efficient genotyping/sequencing assays&#13;
may circumvent the need for cumbersome procedures that were earlier used for fine mapping. A deeper understanding of&#13;
the trait architectures of agricultural significance will be crucial to accelerate crop improvement.</mods:abstract><mods:classification authority="lcc">Plant Breeding</mods:classification><mods:classification authority="lcc">Genetics and Genomics</mods:classification><mods:originInfo><mods:dateIssued encoding="iso8061">2020-02</mods:dateIssued></mods:originInfo><mods:originInfo><mods:publisher>Springer</mods:publisher></mods:originInfo><mods:genre>Article</mods:genre></mods:mods>