http://oar.icrisat.org/id/eprint/11588 2020-09-04T17:01:05Z info:eu-repo/semantics/descriptiveMetadata http://oar.icrisat.org/11588/ Fine mapping and gene cloning in the post-NGS era: advances and prospects Jaganathan, D Bohra, A Thudi, M Varshney, R K Plant Breeding Genetics and Genomics Improvement in traits of agronomic importance is the top breeding priority of crop improvement programs. Majority of these agronomic traits show complex quantitative inheritance. Identification of quantitative trait loci (QTLs) followed by fine mapping QTLs and cloning of candidate genes/QTLs is central to trait analysis. Advances in genomic technologies revolutionized our understanding of genetics of complex traits, and genomic regions associated with traits were employed in marker-assisted breeding or cloning of QTLs/genes. Next-generation sequencing (NGS) technologies have enabled genomewide methodologies for the development of ultra-high-density genetic linkage maps in different crops, thus allowing placement of candidate loci within few kbs in genomes. In this review, we compare the marker systems used for fine mapping and QTL cloning in the pre- and post-NGS era. We then discuss how different NGS platforms in combination with advanced experimental designs have improved trait analysis and fine mapping. We opine that efficient genotyping/sequencing assays may circumvent the need for cumbersome procedures that were earlier used for fine mapping. A deeper understanding of the trait architectures of agricultural significance will be crucial to accelerate crop improvement. Springer 2020-02 Article PeerReviewed application/pdf en http://oar.icrisat.org/11588/1/s00122-020-03560-w.pdf Jaganathan, D and Bohra, A and Thudi, M and Varshney, R K (2020) Fine mapping and gene cloning in the post-NGS era: advances and prospects. Theoretical and Applied Genetics (TSI), 133 (5). pp. 1791-1810. ISSN 0040-5752 https://doi.org/10.1007/s00122-020-03560-w doi:10.1007/s00122-020-03560-w info:eu-repo/semantics/objectFile info:eu-repo/semantics/humanStartPage