eprintid: 11585
rev_number: 10
eprint_status: archive
userid: 3170
dir: disk0/00/01/15/85
datestamp: 2020-09-04 12:47:38
lastmod: 2020-09-04 12:47:38
status_changed: 2020-09-04 12:47:38
type: article
metadata_visibility: show
creators_name: Palit, P
creators_name: Ghosh, R
creators_name: Tolani, P
creators_name: Tarafdar, A
creators_name: Chitikineni, A
creators_name: Bajaj, P
creators_name: Sharma, M
creators_name: Kudapa, H
creators_name: Varshney, R K
creators_gender: Female
creators_gender: Female
creators_gender: Female
creators_gender: Female
icrisatcreators_name: Palit, P
icrisatcreators_name: Ghosh, R
icrisatcreators_name: Tolani, P
icrisatcreators_name: Tarafdar, A
icrisatcreators_name: Chitikineni, A
icrisatcreators_name: Bajaj, P
icrisatcreators_name: Sharma, M
icrisatcreators_name: Kudapa, H
icrisatcreators_name: Varshney, R K
affiliation: ICRISAT (Patancheru)
country: India
title: Molecular and Physiological Alterations in Chickpea under Elevated CO2 Concentrations
ispublished: pub
subjects: s1.1
subjects: s2.13
subjects: s2.8
divisions: CRPS2
divisions: CRPS3
crps: CG1
full_text_status: public
keywords: Climate change, Differentially expressed genes, 
Elevated CO2 concentration, RNA-Seq, Stress pathways, 
Transcriptome
note: Authors are thankful to the Department of Biotechnology,
Department of Science and Technology (DST, Climate
Change Program) of Government of India, and Bill & Melinda
Gates Foundation for supporting this study partially. P.P.
acknowledges Women Scientist Scheme of Department of
Science and Technology, Government of India (SR/WOS-A/
LS-1175/2015). R.K.V. is thankful to the Science & Engineering
Research Board (SERB) of DST, Government of India for providing
the J C Bose National Fellowship (SB/S9/Z-13/2019) and
European Commission for funding the 3-year-long
ERASMUSþ—Capacity Building project entitled
‘Strengthening education, research and innovation for
climate-smart crops in India’ (598797-EPP-1-2018-1-ELEPPKA2-
CBHE-JP). This work has been undertaken as part of
CGIAR Research Program on Grain Legumes & Dryland Cereals.
ICRISAT is a member of CGIAR Research Consortium.
abstract: The present study reports profiling of the elevated carbon
dioxide (CO2) concentration responsive global transcriptome
in chickpea, along with a combinatorial approach for
exploring interlinks between physiological and transcriptional
changes, important for the climate change scenario.
Various physiological parameters were recorded in two
chickpea cultivars (JG 11 and KAK 2) grown in open top
chambers under ambient [380 parts per million (ppm)]
and two stressed/elevated CO2 concentrations (550 and
700 ppm), at different stages of plant growth. The elevated
CO2 concentrations altered shoot and root length, nodulation
(number of nodules), total chlorophyll content and nitrogen
balance index, significantly. RNA-Seq from 12 tissues
representing vegetative and reproductive growth stages of
both cultivars under ambient and elevated CO2 concentrations
identified 18,644 differentially expressed genes including
9,687 transcription factors (TF). The differential regulations
in genes, gene networks and quantitative real-time
polymerase chain reaction (qRT-PCR) -derived expression
dynamics of stress-responsive TFs were observed in both
cultivars studied. A total of 138 pathways, mainly involved
in sugar/starch metabolism, chlorophyll and secondary
metabolites biosynthesis, deciphered the crosstalk operating
behind the responses of chickpea to elevated CO2
concentration.
date: 2020-05
date_type: published
publication: Plant and Cell Physiology (TSI)
volume: 61
number: 8
publisher: Oxford University Press
pagerange: 1449-1463
id_number: doi:10.1093/pcp/pcaa077
refereed: TRUE
issn: 1471-9053
official_url: https://doi.org/10.1093/pcp/pcaa077
related_url_url: https://scholar.google.com/scholar?hl=en&as_sdt=0%2C5&q=10.1093%2Fpcp%2Fpcaa077&btnG=
related_url_type: pub
citation:   Palit, P and Ghosh, R and Tolani, P and Tarafdar, A and Chitikineni, A and Bajaj, P and Sharma, M and Kudapa, H and Varshney, R K  (2020) Molecular and Physiological Alterations in Chickpea under Elevated CO2 Concentrations.  Plant and Cell Physiology (TSI), 61 (8).  pp. 1449-1463.  ISSN 1471-9053     
document_url: http://oar.icrisat.org/11585/1/pcaa077.pdf